Anti-PKC zeta抗體,蛋白激酶C zeta 抗體
英文介紹:Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品名稱:Anti-PKC zeta抗體,蛋白激酶C zeta 抗體
產(chǎn)品編號(hào):BYK-10399R
英文名稱:Anti-PKC zeta
中文名稱:蛋白激酶C zeta 抗體
英文別名:AI098070; aPKCzeta; C80388; EC 2.7.11.13; KPCZ_HUMAN; nPKC zeta; nPKC-zeta; OTTHUMP00000001368; OTTHUMP00000044160; PKC 2; PKC ZETA; PKC2; Pkcz; PKCZETA; PKM-zeta, included; PRKCZ; Protein kinase C zeta; Protein kinase C zeta form; Protein kinase C zeta type; r14-3-3; R74924; zetaPKC; PKC-ZETA; PKC2; nPKC-zeta.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來(lái)電索取說(shuō)明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-PKC zeta抗體,蛋白激酶C zeta 抗體轉(zhuǎn)膜(Transfer)
我們*在Western實(shí)驗(yàn)中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜(二氟化樹(shù)脂膜膜孔徑:0.45um)也可以使用,但硝酸纖維素膜比較脆,在操作過(guò)程中特別是用鑷子夾取等過(guò)程中容易裂開(kāi)。膜的使用請(qǐng)參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標(biāo)準(zhǔn)濕式轉(zhuǎn)膜裝置,可以設(shè)定轉(zhuǎn)膜電壓120V,轉(zhuǎn)膜時(shí)間為30-60分鐘。具體的轉(zhuǎn)膜時(shí)間要根據(jù)目的蛋白的大小而定,目的蛋白的分子量越大,需要的轉(zhuǎn)膜時(shí)間越長(zhǎng),目的蛋白的分子量越小,需要的轉(zhuǎn)膜時(shí)間越短。
在轉(zhuǎn)膜過(guò)程中,特別是高電流快速轉(zhuǎn)膜時(shí),通常會(huì)有非常嚴(yán)重的發(fā)熱現(xiàn)象,把轉(zhuǎn)膜槽放置在冰浴中進(jìn)行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn),通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對(duì)膜進(jìn)行染色,以觀察實(shí)際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍(lán)快速染色液對(duì)完成轉(zhuǎn)膜的SDS-PAGE膠進(jìn)行染色,以觀察蛋白的殘留情況。
封閉(Blocking)
轉(zhuǎn)膜完畢后,立即把蛋白膜放置到預(yù)先準(zhǔn)備好的Western洗滌液中,漂洗1-2分鐘,以洗去膜上的轉(zhuǎn)膜液。從轉(zhuǎn)膜完畢后所有的步驟,一定要注意膜的保濕,避免膜的干燥,否則極易產(chǎn)生較高的背景。
用微型臺(tái)式真空泵吸盡洗滌液,加入Western封閉液,在搖床上緩慢搖動(dòng),室溫封閉60分鐘。對(duì)于一些背景較高的抗體,可以在4℃ 封閉過(guò)夜。
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