產(chǎn)品名稱：DSS, 50MG
貨號(hào)：Pierce 21655
規(guī)格：50MG
Thermo Scientific Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide （NHS） ester at each end of an 8-carbon spacer arm.
NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
DSS is first dissolved in an organic solvent such as DMF or DMSO, then added to the aqueous crosslinking reaction. BS3, the water soluble analog of DSS is also available for applications that require a hydrophilic crosslinker （e.g. to effect cell-surface crosslinking）. DSS and BS3 have essentially identical crosslinking activity toward primary amines.
DSS Highlights
Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
DSS crosslinker is membrane permeable, so intracellular crosslinking can be done
High purity, crystalline DSS can be used to create high-purity conjugates
Applications for DSS
Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
“Fix” protein interactions to allow identification of weak or transient protein interactions
Protein crosslinking to create bioconjugates via single-step reactions
Immobilize proteins onto amine-coated surfaces
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DSS, 50MG 原裝現(xiàn)貨 產(chǎn)品信息
