產(chǎn)品名稱：Sulfo-SMCC
貨號(hào)：Pierce 22322
規(guī)格：50MG
Thermo Scientific Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm （8.3 angstroms）.
Sulfosuccinimidyl-4-（N-maleimidomethyl）cyclohexane-1-carboxylate （Sulfo-SMCC） is a water-soluble, non-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS ester） and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The maleimide groups of Sulfo-SMCC and SMCC are unusually stable up to pH 7.5 because of the cyclohexane bridge in the spacer arm. Because it contains the hydrophilic sulfonyl moiety, Sulfo-SMCC is soluble up to ~10 mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure.
Sulfo-SMCC Highlights
Amine reactive Sulfo-NHS ester crosslinks rapidly with primary amine-containing molecule
Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates
Sulfo-SMCC is water soluble, so crosslinking can be done in physiologic solutions
High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives
Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.
Applications for Sulfo-SMCC
Ideal crosslinker for enzyme labeling of antibodies, both enzyme activity and antibody specificity can be preserved
Create specific bioconjugates via one- or two-step crosslinking reactions
Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens
上海葉舟生物科技有限公司(上海浩然生物技術(shù)有限公司)在為生命科學(xué)領(lǐng)域提供豐富的產(chǎn)品與信息資源方面處于全國(guó)*地位，公司提供的產(chǎn)品涵蓋了二十多個(gè)國(guó)家近五十萬(wàn)種產(chǎn)品，而且產(chǎn)品的數(shù)量與信息在不斷的增長(zhǎng)和更新，公司提供的產(chǎn)品能夠使生命科學(xué)工作者加快對(duì)生物化學(xué)，分子生物學(xué)，細(xì)胞生物學(xué)以及蛋白質(zhì)組學(xué)研究的認(rèn)知，以及分子診斷和臨床醫(yī)學(xué)領(lǐng)域的應(yīng)用。
為方便客戶檢索資料，公司建立了數(shù)據(jù)強(qiáng)大的網(wǎng)站:
為方便客戶與公司交流，公司設(shè)立了在線服 QQ:470003480 496799915
為方便客戶訂購(gòu)產(chǎn)品，公司設(shè)立了直線電話: 021- 51870956 54046790
生物試劑
Sulfo-SMCC 原裝現(xiàn)貨 產(chǎn)品信息
