HTB-148 H4 人腦神經(jīng)膠質瘤細胞 的詳細介紹

HTB-148 H4 人腦神經(jīng)膠質瘤細胞

ATCC® Number:	HTB-148™	Price:	$338.00
Designations: H4 Depositors:  J Riggs Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens （human） Morphology: epithelial Source: Organ: brain Disease: neuroglioma Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.   Applications: transfection host （Roche FuGENE® Transfection Reagents） Tumorigenic: No DNA Profile （STR）: Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 11,12 D5S818: 10,12 D7S820: 8,11 THO1: 7,9 TPOX: 8,11 vWA: 14,18 Cytogenetic Analysis: modal number = 73; range = 63 to 78. This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate （24%）. Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del（2）（p2209） and del（9）（p22） and single der（14）t（1;14）（p22;q22）, del（3）（p2501）, del（7）（q3209）, del（10）（p1301）, t（13q17q）, t（3p13q） and at least eight others. The del（5） （p13）, del（7） （q11） and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 1 Age: 37 years Gender: male Ethnicity: Caucasian Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Subculturing: Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:15 is recommended Medium Renewal: 2 to 3 times per week Preservation: Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002 recommended serum:ATCC 30-2020 References: 22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed: 4544026 22907: Day RS, Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. PubMed: 450131

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iec-6 大鼠小腸隱窩上皮細胞
馬-達二氏犬腎細胞系 MDCK （NBL-2） CRL-34
鴨子胚胎成纖維細胞　 Duck embryo　 CCL-141
小鼠胚胎成纖維細胞 3T3-L1 CCL-173
人結腸直腸腺癌 COLO 320DM CCL-220
人結腸直腸腺癌 NCI-H508 CRL-253
人結腸直腸腺癌 COLO 320HSR CCL-220.1
人B淋巴細胞 Ramos（RA1） CRL-1596
人淋巴母細胞 T2（174×CEM.T2） CRL-1992
大鼠心肌細胞 H9C2（2-1） CRL-1446
豬鼻甲黏膜成纖維細胞 PT-K75 CRL-2528
小鼠胰腺癌細胞 LTPA CRL-2389
大鼠胰腺癌細胞 DSL-6A/C1  CRL-2132
小鼠骨髓瘤B淋巴細胞 SP2/MIL-6 CRL-2016
大鼠腦間質細胞 DI TNC1 CRL-2005
小鼠肌原細胞 C2C12 CRL-1772
鵪鶉纖維肉瘤細胞 QT6 CRL-1708

HTB-148 H4 人腦神經(jīng)膠質瘤細胞
大鼠回腸細胞 IEC-18 CRL-1589
非洲綠猴腎細胞 VERO C1008 [Vero 76, clone E6, Vero E6]  CRL-1586
人成纖維細胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮細胞 WPE-int CRL-2888
人正常前列腺成肌纖維細胞 WPMY-1 CRL-2854
人胰腺導管腺癌細胞 PL45 CRL-2558
人胰腺癌細胞 Panc 05.04 CRL-2557
人胰腺癌細胞 Panc 04.03  CRL-2555
人胰腺癌細胞 Panc 02.03 CRL-2553
人胰腺癌細胞 Panc 08.13 CRL-2551
人胰腺癌細胞 Panc 03.27 CRL-2549
人胰腺癌細胞 Panc 10.05 CRL-2547
人肝癌細胞 SNU-398  CRL-2233
人成神經(jīng)瘤細胞-骨髓 SK-N-DZ CRL-2149
人胰腺癌細胞 HPAC CRL-2119
人胰腺癌細胞 HPAF-II CRL-1997
人黑色素瘤細胞 A375.S2 CRL-1872
人臍靜脈內皮細胞 HUV-EC-C CRL-1730
人黑素瘤細胞 WM-266-4 CRL-1676
人膀胱癌細胞 HT-1376  CRL-1472
人正常前列腺上皮細胞 RWPE-2 CRL-11610

人正常前列腺上皮細胞 RWPE-1 CRL-11609
雞胚胎成纖維細胞 UMNSAH/DF-1 CRL-12203
人乳腺癌細胞 MDA-MB-231 HTB-26
小鼠巨噬細胞 RAW 264.7 TIB-71
人橫紋肌肉瘤細胞 A-204 HTB-82
人胰腺癌細胞 Capan-2  HTB-80
人胰腺癌細胞 Capan-1 HTB-79
人乳腺導管癌細胞 MDA-MB-435S HTB-129
人胃癌細胞 Hs 746T HTB-135
人肺腺癌細胞 NCI-H441 [H441] HTB-174
人骨髓瘤細胞 U266  TIB-196
人結腸癌細胞 NCI-H716 [H716] CCL-251
人多形性惡性膠質瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細胞瘤細胞 BE（2）-C CRL-2268
人腎透明細胞癌細胞 Caki-2 HTB-47
人皮膚成纖維細胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤細胞系
CRL-5826 NCI-H226 人肺鱗癌細胞系
Tca-8113 人舌鱗癌細胞
MA-891 小鼠乳腺癌高轉移細胞
U14 小鼠宮頸癌細胞
CRL-9518 L5178Y 小鼠淋巴瘤細胞
HTB-112 HEC-1-A 人子宮內膜腺癌細胞
HTB-113 HEC-1-B 人子宮內膜腺癌細胞

AN3 CA 人子宮內膜腺癌細胞
ROS1728 大鼠成骨細胞
CRL-6323 B16F1 小鼠黑色素瘤細胞
HTB-58 SK-MES-1 人肺鱗癌細胞
CCL-127 IMR-32 人神經(jīng)母細胞瘤細胞
HTB-79 Capan-2 人胰腺癌細胞
BALL-1 人B淋巴細胞急性白血病細胞
CRL-2922 Eayh926 人臍靜脈細胞融合細胞
WB-F344  大鼠肝干細胞
CRL-1715 TM4  正常小鼠睪丸細胞
CRL-2539 4T1 小鼠乳腺癌細胞
CRL-1442 BRL-3A 大鼠肝細胞 
BRL 大鼠肝細胞
RSC96大鼠雪旺細胞
Isk 人子宮內膜癌細胞
HTB-113 hec-1-b 人子宮內膜腺癌細胞
PLA-802?人惡性橫紋肌肉瘤
Pt-K2 袋鼠腎細胞
BS-C-1 非洲綠猴腎細胞
A498人腎癌細胞              
3AO人卵巢癌細胞

iec-6 大鼠小腸隱窩上皮細胞
u87MG 人腦星形膠質母細胞瘤
Renca  小鼠腎癌細胞
nci-h929 骨髓瘤細胞
P388小鼠白血病細胞
opm-2 骨髓瘤細胞
MDA-MB-435 人乳腺癌高轉移細胞
KU812 人外周血嗜堿性白細胞
NIH:OVCAR-3 人卵巢癌細胞
SW 1990人胰腺癌細胞系
S180人結腸腺癌細胞
HuTu-80十二指腸腺癌
RL95-2 人子宮內腺癌細胞
NCI-H446 人小細胞肺癌細胞
MSTO-211H 人肺癌細胞
H4-Ⅱe 大鼠肝癌細胞系
HLF 人肺成纖維樣細胞
LS 180人結腸腺癌細胞
HuTu-80十一指腸腺癌
ST豬睪丸細胞系
REH人急性非B非T淋巴細胞白血病
NCI-H520 人肺鱗癌細胞
RKO 人結腸腺癌細胞
293A 人胚腎細胞
RK-13 兔腎細胞系