Super Script Ⅱ陰性逆轉(zhuǎn)錄酶,SuperScript II Reverse Transcriptase
英文名稱：SuperScript II Reverse Transcriptase
級別：BR
來源：Purified from E. coli expressing the pol gene of M-MLV （1,2）, mutagenized to reduce the RNase H activity
濃度：200u/ul
活力定義：One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly（A）•oligo（dT）12-18 as template•primer （3）.
Unit Reaction Conditions：50 mM Tris-HC1 （pH 8.3）, 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly（A）, 0.1 mM oligo（dT）12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃
Performance and Quality Testing：SDS-PAGE purity；endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
Enzyme Function：RNA dependent DNA Polymerase
Final Product Size：12.3 kb or less
Number of Reactions：10 Reactions
Optimal Reaction Temperature：42℃
Sensitivity：Medium
Template：ssRNA；ssDNA；RNA-DNA Hybrid
產(chǎn)品描述：SuperScript® II Reverse Transcriptase （RT） is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like SuperScript® RT, SuperScript® II RT has reduced RNase H activity. Unlike SuperScript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics, including：Greater first-strand cDNA yields；More full-length cDNA synthesis；Full activity at 42℃
性狀：懸浮液
用途：生化研究。
保存：-20℃
Super Script Ⅱ陰性逆轉(zhuǎn)錄酶,SuperScript II Reverse Transcriptase 質(zhì)量品牌保證，價格公正，傾力為國內(nèi)國外生命科學(xué)研究人員提供*的產(chǎn)品。咨詢！
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