大鼠*(cAMP)ELISA試劑盒
英文名稱:Rat cyclic adenosine monophosphate,cAMP ELISA 試劑盒
包裝:96T/48T
保存:2-8℃
檢測(cè)原理: 采用雙抗體夾心ABC-ELISA法
實(shí)驗(yàn)方法:: :
酶聯(lián)免疫法/酶免法(ELISA)
產(chǎn)品用途:科研ELISA檢測(cè)試劑盒.本公司可提供實(shí)驗(yàn)代測(cè)服務(wù).
服務(wù)承諾:工作時(shí)間內(nèi)免費(fèi)的技術(shù)咨詢和指導(dǎo),為客戶提供來(lái)樣檢測(cè)服務(wù),zui大限度實(shí)驗(yàn)結(jié)果的有效性。
大鼠*(cAMP)ELISA試劑盒 洗滌
洗滌在ELISA過(guò)程中雖不是一個(gè)反應(yīng)步驟,但卻也決定著實(shí)驗(yàn)的成敗。ELSIA就是靠洗滌來(lái)達(dá)到分離游離的和結(jié)合的酶標(biāo)記物的目的。通過(guò)洗滌以清除殘留在板孔中沒(méi)能與固相抗原或抗體結(jié)合的物質(zhì),以及在反應(yīng)過(guò)程中非特異性地吸附于固相載體的干擾物質(zhì)。聚苯乙烯等塑料對(duì)蛋白質(zhì)的吸附是普遍性的,而在洗滌時(shí)又應(yīng)把這種非特異性吸附的干擾物質(zhì)洗滌下來(lái)??梢哉f(shuō)在ELISA操作中,洗滌是zui主要的關(guān)鍵技術(shù),應(yīng)引起操作者的高度重視,操作者應(yīng)嚴(yán)格按要求洗滌,不得馬虎。“”
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