潮霉素B試劑實(shí)驗(yàn)分析說明

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潮霉素B試劑實(shí)驗(yàn)分析說明

級(jí)別：Ultra pure grade
含量：≥80%

濃度：50mg/ml

Working concentration: 50～1000μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used
LD50：6 mg/kg in mouse (i.v.)；13 mg/kg in guinea pig (i.p.)；63 mg/kg in rat (i.p.)
性狀：液體，50 mg/ml, in PBS (phosphate-buffered saline), sterile-filtered
用途：生化研究。是一種氨基糖苷類抗生素，通過阻斷多肽的合成，抑制多肽延長(zhǎng)，用于原核和真核細(xì)胞的篩選。Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

潮霉素B試劑實(shí)驗(yàn)分析說明