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當(dāng)前位置:上海滬震生物科技有限公司>>技術(shù)文章>>人表達于SiSo細胞系的受體結(jié)合型腫瘤抗原ELISA kit
人表達于SiSo細胞系的受體結(jié)合型腫瘤抗原(RCAS1)ELISA kit產(chǎn)品類型:
ELISA Kit
產(chǎn)品名稱:
人表達于SiSo細胞系的受體結(jié)合型腫瘤抗原(RCAS1)ELISA kit
英文名稱:
Human receptor-binding cancer antigen expressed on SiSo cells,RCAS1 ELISA kit
貨號:
HZ-E12153h
別名:
EB9, PDAF, RCAS1, cancer associated surface antigen|estrogen receptor binding site associated antigen 9
規(guī)格:
96T
種屬:
Human
待測物名稱:
estrogen receptor binding site associated, antigen, 9
縮寫:
EBAG9
蛋白功能1:
Apoptosis/Autophagy
蛋白功能3:
Apoptosis
檢測范圍:
0.78 U/ml-50 U/ml
靈敏度:
0.195 U/ml
反應(yīng)時間:
1-5h
所需樣本體積:
50-100ul
檢測波長:
450 nm
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RCAS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RCAS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCAS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCAS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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