動物細(xì)胞培養(yǎng)的條件

一般來說，人肺腺癌細(xì)胞 PAa細(xì)胞體外培養(yǎng)需要滿足以下條件

1、無菌、無毒的環(huán)境：首先應(yīng)保證被培養(yǎng)的細(xì)胞處于無菌、無毒的環(huán)境，即對培養(yǎng)液和所有的培養(yǎng)用具進(jìn)行無菌處理。通常還要在培養(yǎng)液中添加一定量的抗生素以防培養(yǎng)過程中的污染。

2、營養(yǎng)物質(zhì)：細(xì)胞體外培養(yǎng)所需營養(yǎng)物質(zhì)與體內(nèi)基本相同，需要有葡萄糖、氨基酸、促生長因子、無機(jī)鹽、微量元素等。通常還需加入血清、血漿等一些天然成分。

3、溫度和pH：細(xì)胞體外培養(yǎng)的適宜溫度一般與動物的體溫相近。多數(shù)細(xì)胞生存的適宜pH為7.2～7.4。

4、氣體環(huán)境：人肺腺癌細(xì)胞 PAa細(xì)胞培養(yǎng)所需氣體主要有O2和CO2，O2是細(xì)胞代謝所必需的，CO2的主要作用是維持培養(yǎng)液的pH。進(jìn)行細(xì)胞培養(yǎng)時，通常采用培養(yǎng)皿或松蓋培養(yǎng)瓶，將其置于含95%空氣加5% CO2的混合氣體的培養(yǎng)箱中進(jìn)行培養(yǎng)。

培養(yǎng)的過程：

下面介紹動物細(xì)胞培養(yǎng)與植物組織培養(yǎng)的比較

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CEA544Ga    雞免疫球蛋白G(IgG)ELISA試劑盒    ELISA Kit for Immunoglobulin G (IgG)
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SEA064Ga    雞白介素18(IL18)ELISA試劑盒    ELISA Kit for Interleukin 18 (IL18)
SEA033Ga    雞干擾素α(IFNα)ELISA試劑盒    ELISA Kit for Interferon Alpha (IFNa)
SEA222Ga    雞干擾素β(IFNβ)ELISA試劑盒    ELISA Kit for Interferon Beta (IFNb)
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SEB824Ga    雞核因子κB(NFκB)ELISA試劑盒    ELISA Kit for Nuclear Factor Kappa B (NFkB)
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SEA106Ga    雞神經(jīng)營養(yǎng)因子3(NT3)ELISA試劑盒    ELISA Kit for Neurotrophin 3 (NT3) 
SEA002Ga    雞白細(xì)胞激活黏附因子(ALCAM)ELISA試劑盒    ELISA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM) 
SEA112Ga    雞β細(xì)胞素(βTC)ELISA試劑盒    ELISA Kit for Betacellulin (bTC) 
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SEA060Ga    雞白介素13(IL13)ELISA試劑盒    ELISA Kit for Interleukin 13 (IL13) 
SEA061Ga    雞白介素15(IL15)ELISA試劑盒    ELISA Kit for Interleukin 15 (IL15) 
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