上皮鈣粘附分子抗體E cadherin相關(guān)標(biāo)記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin   Cy3   Cy5  
Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC 
英文名稱    E cadherin
中文名稱    上皮鈣粘附分子抗體
別    名    E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; CADH1_HUMAN.
研究領(lǐng)域    腫瘤  細(xì)胞生物  免疫學(xué)  細(xì)胞粘附分子  細(xì)胞表面分子  上皮細(xì)胞  
抗體來(lái)源    Rabbit
克隆類型    Polyclonal
上皮鈣粘附分子抗體E cadherin交叉反應(yīng)    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, 
產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    20/80kDa
細(xì)胞定位    細(xì)胞膜 
性    狀    Lyophilized or Liquid
濃    度    1mg/1ml
免 疫 原    KLH conjugated synthetic peptide derived from human E-cadherin (841-882aa)
亞    型    IgG
純化方法    affinity purified by Protein A
儲(chǔ) 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹    background:
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

Function:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. 
E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subunit:
Homodimer.

Subcellular Location:
Cell junction. Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Non-neural epithelial tissues.

Post-translational modifications:
During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.

DISEASE:
Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. 
Defects in CDH1 are a cause of gastric cancer [MIM:137215]; also known as hereditary familial diffuse gastric cancer (HDGC). 
Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089]. 
Defects in CDH1 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.

Similarity:
Contains 5 cadherin domains.

Gene ID:
999

Database links:
Entrez Gene: 999 Human
Entrez Gene: 12550 Mouse
Entrez Gene: 83502 Rat
Omim: 192090 Human
SwissProt: P12830 Human
SwissProt: P09803 Mouse
SwissProt: Q9R0T4 Rat
Unigene: 461086 Human
Unigene: 35605 Mouse
Unigene: 1303 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

細(xì)胞粘附蛋白（Call Adhesion Protein）
E-chaherin是研究較多的同質(zhì)粘附分子。E-cadherin的表達(dá)與惡性腫瘤的分化程度、侵襲力、轉(zhuǎn)移負(fù)相關(guān)與預(yù)后正相關(guān)，E-cadherin的低表達(dá)和不穩(wěn)定表達(dá)可促進(jìn)轉(zhuǎn)移的發(fā)生。近年來(lái)已成為腫瘤細(xì)胞侵襲和轉(zhuǎn)移研究的熱點(diǎn)之一。