丁酰*酯酶應(yīng)用技術(shù)CAS號：9001-08-5

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丁酰*酯酶應(yīng)用技術(shù)

分子量：～440000

級別：BR
活力：≥4units/mg 

產(chǎn)品描述：It catalyzes the hydrolysis of a number of choline esters according to the following reaction：Acylcholine+H2O to choline+acid.It is a tetrameric glycoprotein with four subunits of equal size,each having a molecular weight of 110KDa.Butyryl cholinesterase hydrolyzes butyrylcholine four times more rapidly than acetylcholine.It is inhibited by 10-5 M physostigmine，numerous organophosphate esters,the carbamate derivates and quaternary ammonium saltsunit hydrolyzes 1umole of acetylchoine per minute at 25℃, pH7.4
性狀：淡褐色粉末。*酯酶有2種：AChE和丁酰*酯酶(BuChE)。兩者的基因結(jié)構(gòu)、氨基酸序列及空間三維結(jié)構(gòu)均顯示了很高的同源性。二者的主要差異在于，AChE水解乙酰*(ACh)和乙酰硫代*(ATCh)比丙酰*(PrCh)快，對丁酰*(BuCh)和丁酰硫代*(BuTCh)沒有明顯的水解作用。丁酰*酯酶與乙酰*酯酶的顯色反應(yīng)原理是*一樣的。丁酰*酯酶具有靈敏度更高的優(yōu)點(diǎn)
用途：生化研究。是一類糖蛋白，水解乙酸*。是生物神經(jīng)傳導(dǎo)中的一種關(guān)鍵性酶，同時又是有機(jī)磷和氨基甲酸酯殺蟲劑的靶標(biāo)。 
保存：2～8℃

丁酰*酯酶應(yīng)用技術(shù)