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Leptin Receptor Chicken/重組雞瘦素受體 產(chǎn)品編號(hào)：CYT-509

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Leptin Receptor Chicken/重組雞瘦素受體 產(chǎn)品編號(hào)：CYT-509
產(chǎn)品規(guī)格：10μg/50μg/1mg

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Leptin Receptor Chicken/重組雞瘦素受體

產(chǎn)品編號(hào)：CYT-509
產(chǎn)品規(guī)格：10μg/50μg/1mg
 

Synonyms OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor.
Description Leptin Binding Domain Chicken Recombinant also called Leptin Receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Chicken Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 420-626 of chicken leptin receptor.
The Leptin Binding Domain is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation The protein was lyophilized from a concentrated （0.2 to 0.5 mg/ml） solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl and 10-fold （w/w） excess of glycine.
Solubility It is recommended to reconstitute the lyophilized Leptin Receptor Chicken in sterile water at pH 8-9, not less than 100μg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability Leptin Receptor Chicken should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin Receptor Chicken mg/ml and up to 40.5 mg/ml mM and filter sterilization chLBD can be stored at 4°C for several months. Sterile solutions at 0.5 mg/ml or less are stable at +4°C for several months.
Please prevent freeze-thaw cycles.
Purity Greater than 99.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile Biological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.

2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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