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人纖維連接蛋白/Human Fibronectin產(chǎn)品編號：PRO-448
產(chǎn)品規(guī)格：200μg/1mg/10mg
| Catalogue Number | PRO-448 |
| Introduction | Plasma fibronectin level is elevated in severe coronary artery disease. Increased plasma fibronectin levels are related with venous thromboembolism （VTE） particularly in males, and extend the probable association between biomarkers and risk factors for arterial atherothrombosis and VTE. Fibronectin plays a role in several cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin consists in two main forms: 1） as an insoluble glycoprotein dimer that serves as a linker in the etracellular matrix and 2） as a soluble disulphide linked dimer found in the plasma. The plasma form is produced by hepatocytes, and the ECM form is synthesized by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin alos takes part as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. Fibronectin organizes cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound Fibronectin receptors on cell surfaces. Molecular Weight 450kDa. |
| Source | Human Plasma. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The Fibronectin was lyophilized from a concentrated 1mg/ml solution containing 0.05M Tris Cl pH-7.5 and 0.1M NaCl. |
| Solubility | Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water.
Let stand 1-2 hours. |
| Stability | Store the lyophilized fibronectin at 4°C. Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C.
Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Cell Culture Application | Fibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50ug/ml. The plates are treated with the solution, at room temperature 1-2 hours, the supernatant removed and the plates rinsed with fresh media or buffer. Growth media is then added and the coated plates seeded with a suspension of freshly harvested cells. Care should be taken to inactivate serum proteases used in the preparation of the cells, since fibronectin is very sensitive to proteolysis. Optimum concentration of fibronectin depends on the types of cell and the particular surface used. Generally, use 1-5ug/cm2 of growth surface. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
中文翻譯，僅供參考：
產(chǎn)品描述:
在患有嚴(yán)重冠狀動(dòng)脈疾病患者體內(nèi)，血漿纖維連接蛋白含量升高。血漿纖維連接蛋白含量的升高與靜脈血栓栓塞（VTE）有關(guān)，尤其是男性。它可以作為動(dòng)脈粥樣硬化和靜脈血栓栓塞的生物標(biāo)志物和危險(xiǎn)因素。纖維連接蛋白在細(xì)胞方面的作用包括：組織修復(fù)，胚胎發(fā)育，血液凝固，細(xì)胞遷移/粘合。纖維連接蛋白包括兩種主要形式：1）作為一種不溶性糖蛋白二聚體，與細(xì)胞外連接。2）作為一種可溶性二硫化物，連接血漿中的二聚體。血漿是由肝細(xì)胞合成的，細(xì)胞外基質(zhì)是由成纖維細(xì)胞，軟骨細(xì)胞，內(nèi)皮細(xì)胞，巨噬細(xì)胞，以及某些上皮細(xì)胞合成的。纖維連接蛋白作為一種細(xì)胞黏附分子將細(xì)胞錨定到膠原蛋白或蛋白多糖基質(zhì)上。纖維連接蛋白通過結(jié)合到不同的細(xì)胞外組分以及結(jié)合到細(xì)胞表面膜結(jié)合纖維連接蛋白受體上，從而與細(xì)胞外基質(zhì)發(fā)生細(xì)胞相互作用。分子量為450kDa。
來源:
人血漿
貨號: PRO-448
物理性質(zhì):
凍干無菌過濾白色（凍干）粉.
劑型:
纖維連接蛋白是從濃度為1mg/ml，含0.05M的Tris HCl（ pH值7.5）和0.1M的氯化鈉溶液中凍干而來的。
溶解性:
純化的纖維連接蛋白在重懸后很容易形成不溶性聚合物。我們建議使用離液序列高的試劑如尿素，在室溫下重懸1mg的纖維連接蛋白，使其濃度達(dá)到0.2mg/ml。
靜置1-2小時(shí)。
穩(wěn)定性:
凍干的淋巴細(xì)胞分離液雖然在室溫可以貯存1星期，貯存在-18°C以下。
重懸的纖維連接蛋白4°C可以保存2-7天。*保存要貯存在-18°C以下。
不要反復(fù)凍融。
純度:
SDS-PAGE檢測，純度大于95.0% 。
細(xì)胞培養(yǎng)應(yīng)用:
纖維連接蛋白在誘導(dǎo)細(xì)胞黏附到各種表面包括塑料，玻璃組織培養(yǎng)器皿，培養(yǎng)皿，蓋玻片，微載體磁珠，等非常有用。纖維連接蛋白能夠使細(xì)胞在低血清培養(yǎng)基中生長。一般來說，應(yīng)該用無菌生理鹽水或無血清培養(yǎng)基重懸纖維連接蛋白，使稀釋的濃度保持在10-50ug/ml。用溶液室溫處理培養(yǎng)皿1-2小時(shí)，然后棄掉液體，用新鮮培養(yǎng)基或者緩沖液沖洗培養(yǎng)皿。加入生長培養(yǎng)基，然后在包被的培養(yǎng)皿中接種新鮮的細(xì)胞懸液。應(yīng)注意要在培養(yǎng)的細(xì)胞中加入滅活的血清蛋白酶，因?yàn)槔w維連接蛋白很容易水解。*的纖維連接蛋白濃度取決于細(xì)胞類型和使用表面積。一般來說，使用生長面積為1-5ug/cm2。
用法:
ProSpec的產(chǎn)品只能用于科學(xué)研究。ProSpec的產(chǎn)品不能用于藥物，農(nóng)業(yè)或者殺蟲產(chǎn)品，食物添加劑或者家用化學(xué)藥品。
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