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D0047植物血球凝集素PHA-M
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產(chǎn)品介紹
Description
Analysis Note Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.
Biochem/physiol Actions PHA consists of two molecular species, an erythroagglutinin （PHA-E） which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin （PHA-L） which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
Properties
form lyophilized powder
potency <40 μg per mL agglutination activity
composition Protein, 60-80% biuret
total impurities salt, free
storage temp. 2-8°C
Safety
Safety Statements 22-24/25
RTECS TJ3470000
