Mouse monoclonal antibody to GAPDH
Catalog Number :
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, thereversible oxidative phosphorylation of glyceraldehyde-3-phosphatein the presence of inorganic phosphate and nicotinamide adenine dinucleotide （NAD）. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export （1）. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease （2）. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Specificity : · anti-GAPDH is a mouse monoclonal antibody unconjugated
· specific for GAPDH of mouse, rat
· use for western blotting, Elisa, immunoprecipitation and
immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype:IgG
· mol wet:37kDa
· Clone number:3E12
Application : ·Western blotting 1:500-1000
· Immunohistochemistry 1:100-500
· ELISA 1:500-5000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
