Mouse Anti- Klotho抗體說(shuō)明書(shū)，一抗，單抗
FROM: ABCAM
Cat. Number:  Rs-2925R
Quantity size:  0.1ml  （dilute with pH 7.4    0.01 M PBS or diluent of antibody）

Background:  May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D （By similarity）. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Specificity:      •anti-Klotho  is a mouse monoclonal antibody unconjugated
                •specific for Klotho  of  human 
                •epitope mapping near the amino terminus ofKlotho   of  
                   human
                • use for western blotting, elisa, immunoprecipitation    
                   and immunohistochemistry
•  Protein A affinity chromatography purification, purity :>95%
• Isotype: IgG
• mol wt: 82kDa
 Mouse Anti- Klotho抗體說(shuō)明書(shū)，一抗，單抗

Application:     • Western blotting: 1:100-500
• Immunohistochemistry: 1:100-500
• ELISA: 1:500-1000
•  Optimal working dilutions must be determined by the end user.

Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note:  This product as supplied is  intended for research use only, not for use in human, therapeutic or diagnostic applications.
Mouse Anti- Klotho抗體說(shuō)明書(shū)，一抗，單抗