Anti-CK18抗體說明書
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Specificity : · anti- CK18 is a rabbit polyclonal antibody unconjugated
· specific for CK18 of hu, rat, mo, cow, pig
· epitope mapping in the C-terminalse of CK18 of hu, rat, mo, cow, pig
· use for western blotting, elisa, immunoprecipitation and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 34kDa
Application: ·Western blotting 1:100-500
·Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-CK18抗體說明書