From: Santa Cruz

 

Catalog Number: Rs-1500R  

 

Quantity size: 0.1ml （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

 

Background: Cathepsin B（CTSB; APPS;CPSB; Cathepsin B1; APP secretase; Amyloid precursor protein secretase; Cysteine protease; Preprocathepsin B） is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein （APP）. Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene.  

Specificity : · anti- Cathepsin B is a rabbit monoclonal antibody unconjugated

           · specific for Cathepsin B of guinea pig, human, mouse and rat  

           · epitope mapping in the middle of Cathepsin B of guinea pig, human, mouse and rat origin  

           ·  use for western blotting,elisa, immunoprecipitation and immunohistochemistry

· Protein G affinity chromatography purification, purity :>95%

·  Isotype: IgG

·  mol wt:37kDa

 

Application : · Western blotting 1:100-500

            · Immunohistochemistry 1:100-500

            · ELISA 1:500-1000

            · Optimal working dilutions must be determined by the end user

 

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.