小鼠膠原酶II(Collagenase II)ELISA試劑盒
Mouse Collagenase II ELISA Kit
實(shí)驗(yàn)原理：
本試劑盒僅供研究使用，用于測定小鼠血清，血漿及相關(guān)液體樣本中膠原酶II(Collagenase II)的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中小鼠膠原酶II(Collagenase II)水平。用純化的小鼠膠原酶II(Collagenase II)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入膠原酶II(Collagenase II)，再與HRP標(biāo)記的膠原酶II(Collagenase II)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的膠原酶II(Collagenase II)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中小鼠膠原酶II(Collagenase II)濃度。
Principle of the assay
The kit allows for the determination of Collagenase II concentrations in Mouse serum, blood plasma, and other biological fluids.Assay Mouse Collagenase II level in the sample，use Purified Mouse Collagenase II antibody to coat microtiter plate wells, make solid-phase antibody, then add Collagenase II to wells, Combined Collagenase II antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Collagenase II in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司
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