人粘蛋白1(MUC1)酶聯(lián)免疫ELISA試劑盒
實(shí)驗(yàn)原理 ：
本試劑盒用于測定人血清，血漿及相關(guān)液體樣本中粘蛋白1(MUC1)的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中 人粘蛋白1(MUC1)水平。用純化的人粘蛋白1(MUC1)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中加入粘蛋白1(MUC1)，再與HRP標(biāo)記的粘蛋白1(MUC1)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB 顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的粘蛋白1(MUC1)呈正相關(guān)。用酶標(biāo)儀在 450nm 波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中人粘蛋白1(MUC1)的濃度。
Generic Name：Human Mucin 1 (MUC1) ELISA KIT
Purpose:
This kit allows for the determination of MUC1 concentrations in Human serum, blood
plasma, and other biological fluids.
Principle of the assay:
The kit assay Human MUC1 level in the sample，use Purified Human MUC1 to coat microtiter plate wells, make solid-phase antibody, then add MUC1 to wells, Combined MUC1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MUC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA KIT供應(yīng)商：上海華壹生物科技有限公司
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