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Mouse anti-hemoglobin
(monoclonal antibody)
Quantity size : 0.2ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
Background: Hemoglobin is involved in oxygen transport from the lung to the various
peripheral tissues. The alpha (HBA) and beta (HBB) loci determine the structure of the 2
types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer
consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell
anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of
detectable beta globin causes beta plus thalassemia.
Specificity : · anti- Hemoglobin is a mouse monoclonal antibody unconjugated
· specific for Hemoglobin of human
·use for western blotting, Elisa, immunoprecipitation and
immunohistochemistry
·Protein G affinity chromatography purification, purity :>95%
·Isotype: IgG
· mol wet:67kDa
Application : · Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-5000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 oC for one year. Avoid repeated freeze/thaw cycles. The
lyophilized antibody is stable at room temperature for at least one month and for greater
than a year when kept at -20oC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent
of antibody, the antibody is stable for at least two weeks at 2-4 oC.
Important Note: This product as supplied is intended for research use only, not for use in
human, therapeutic or diagnostic applications.
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