上海豐翔生物科技有限公司

Rabbit  Anti-Insulin

Catalog Number :HZ23155       Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）

Background:    Insulin is a secreted peptide hormone that elicits metabolic effects such as increases in glucose uptake and glycogen synthesis leading to a decrease in blood glucose concentration. Insulin is first formed as a precursor molecule,  preproinsulin, which is later cleaved to proinsulin and finally to the mature Insulin hormone. Mature Insulin consists of 51 amino acids, contained within an A chain and a B chain that are connected by two disulfide bridges. It increases cell permeability to monosaccharides, amino acids and fatty acids Insulin is secreted by the pancreas at basal levels in the absence of exogenous stimuli, with secretion increasing in response to glucose. Insulin action is effected by the binding of Insulin to cell-surface receptors on the target cell membrane. Defects of Insulin are the cause of hyperproinsulinemia and of type 2 diabetes mellitus.

pecificity :   

–        Anti- Insulin is a rabbit polyclonal antibody unconjugated                

–         specific for Insulin of human, mouse, rat          

–         use for western blotting,elisa,immunoprecipitation and immunohistochemistry

–         Protein G affinity chromatography purification, purity :>95%

–         Isotype:  IgG

–         mol wt:  12kDa

Application :  

–        Western blotting 1:100-500                

–         Immunohistochemistry 1:100-500                 

–         ELISA 1:500-1000                  

–         Optimal working dilutions must  be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

 Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

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