小鼠5羥色胺(5-HT)ELISA試劑盒子科生物現(xiàn)貨供應(yīng),凡購(gòu)買(mǎi)子科生物子科生物任何一款ELISA酶聯(lián)免疫分析檢測(cè)試劑盒,都可以享受免費(fèi)代測(cè)服務(wù),外地客戶(hù)可以由技術(shù)老師的指導(dǎo)下正確放置好樣本的保存方式后快遞郵寄到我公司技術(shù)部,本地客戶(hù)可以享受免費(fèi)上門(mén)取樣服務(wù)!
●產(chǎn)品名稱(chēng):小鼠5羥色胺(5-HT)ELISA試劑盒
●英文名稱(chēng):Mouse 5-Hydroxytryptamine,5HT ELISA Kit
●貨號(hào):ZK-M4927
●規(guī)格: 96T/48T
●品牌:子科生物ZIKER
●反應(yīng)時(shí)間: 1-5h
●所需樣本體積: 50-100ul
●檢測(cè)波長(zhǎng): 450 nm
●用途: For research use only. Not for diagnostic use.
●特點(diǎn):靈敏性高、特異性強(qiáng)、重復(fù)性好
●服務(wù):子科生物所有的elisa試劑盒均可免費(fèi)代測(cè),全程Elisa實(shí)驗(yàn)技術(shù)指導(dǎo)。
●運(yùn)輸:全國(guó)各地快遞免費(fèi)送貨上門(mén),低溫運(yùn)輸,用冰袋加泡沫保溫盒包裝,確保產(chǎn)品運(yùn)輸狀態(tài)下也處于低溫狀態(tài)。
深圳子科生物所有ELISA試劑盒均需要經(jīng)過(guò)質(zhì)檢合格后才允許出庫(kù),能有效控制產(chǎn)品質(zhì)量,廣大客戶(hù)可以放心購(gòu)買(mǎi)。且子科生物所有ELISA試劑盒里的抗體均采用進(jìn)口品牌抗體,能有效保證產(chǎn)品的高效靈敏性,且我公司研發(fā)人員有豐富的研發(fā)經(jīng)驗(yàn),*的儀器設(shè)備,*的實(shí)驗(yàn)技術(shù),讓我公司產(chǎn)品優(yōu)于同等廠家系列產(chǎn)品,是您的*廠家。
小鼠5羥色胺(5-HT)ELISA試劑盒實(shí)驗(yàn)原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標(biāo)抗體的微孔中依次加入標(biāo)本或標(biāo)準(zhǔn)品、生物素化的抗該指標(biāo)抗體、HRP標(biāo)記的親和素,經(jīng)過(guò)*洗滌后用底物TMB顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的該指標(biāo)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),計(jì)算樣品濃度。
標(biāo)本的采集及保存
1. 血清:全血標(biāo)本請(qǐng)于室溫放置2小時(shí)或4℃過(guò)了夜后于1000 x g離心20分鐘,取上清即可檢測(cè),或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標(biāo)本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
3.細(xì)胞培養(yǎng)物上清或其它生物標(biāo)本:1000 x g離心20分鐘,取上清即可檢測(cè),或?qū)?biāo)本放于-20℃或-80℃保存,但應(yīng)避免反復(fù)凍融。
注:標(biāo)本溶血會(huì)影響zui后檢測(cè)結(jié)果,因此溶血標(biāo)本不宜進(jìn)行此項(xiàng)檢測(cè)。
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