產(chǎn)品名稱(chēng)：A549細(xì)胞,人肺癌細(xì)胞

產(chǎn)品簡(jiǎn)介：上海博研生物*的ATCC細(xì)胞供應(yīng)商，提供A549細(xì)胞,人肺癌細(xì)胞的報(bào)價(jià)，咨詢(xún)，技術(shù)服務(wù)，咨詢(xún)選購(gòu)。
產(chǎn)品庫(kù)存：現(xiàn)貨
產(chǎn)品價(jià)格：詢(xún)價(jià)
供 應(yīng) 商：ATCC

描述：該細(xì)胞系由D.J.Griad通過(guò)肺癌組織移植培養(yǎng)建系，患者為58歲白人男性。 A549能通過(guò)胞苷二磷酸*途徑合成含有高含量不飽和脂肪酸的*。

動(dòng)物種別：人

組織來(lái)源：肺癌

形態(tài)：上皮細(xì)胞

培養(yǎng)基和添加劑：F12K培養(yǎng)基(SIGMA,貨號(hào)N3520，添加NaHCO3 2.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

技術(shù)相關(guān)問(wèn)答：
1. 如何選用特殊細(xì)胞系培養(yǎng)基？培養(yǎng)某一類(lèi)型細(xì)胞沒(méi)有固定的培養(yǎng)條件。在MEM中培養(yǎng)的細(xì)胞，很可能在DMEM或M199中同樣很容易生長(zhǎng)?？傊?，*MEM做粘附細(xì)胞培養(yǎng)、RPMI-1640做懸浮細(xì)胞培養(yǎng)是一個(gè)好的開(kāi)始。2. 何時(shí)須更換培養(yǎng)基？視細(xì)胞生長(zhǎng)密度而定， 或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間，按時(shí)更換培養(yǎng)基即可。 3. 可否使用與原先培養(yǎng)條件不同之培養(yǎng)基？不能。每一細(xì)胞株均有其特定使用且已適應(yīng)之細(xì)胞培養(yǎng)基， 若驟然使用和原先提供之培養(yǎng)條件不同之培養(yǎng)基， 細(xì)胞大都無(wú)法立即適應(yīng)， 造成細(xì)胞無(wú)法存活。4.可否使用與原先培養(yǎng)條件不同之血清種類(lèi)？不能。血清是細(xì)胞培養(yǎng)上一個(gè)極為重要的營(yíng)養(yǎng)來(lái)源，所以血清的種類(lèi)和品質(zhì)對(duì)于細(xì)胞的生長(zhǎng)會(huì)產(chǎn)生*的影響。來(lái)自不同物種的血清， 在一些物質(zhì)或分子的量或內(nèi)容物上都有所不同，血清使用錯(cuò)誤常會(huì)造成細(xì)胞無(wú)法存活?；竟残?
1、所有的細(xì)胞表面均有由磷脂雙分子層與鑲嵌蛋白質(zhì)及糖被構(gòu)成的生物膜，即細(xì)胞膜。
2、所有的細(xì)胞都含有兩種核酸：即DNA與RNA。
3、作為遺傳信息復(fù)制與轉(zhuǎn)錄的載體。
4、作為蛋白質(zhì)合成的機(jī)器—核糖體，毫無(wú)例外地存在于一切細(xì)胞內(nèi)，核糖體，是蛋白質(zhì)合成的機(jī)器，在細(xì)胞遺傳信息流的傳遞中起重要作用。
5、所有細(xì)胞的增殖都以一分為二的方式進(jìn)行分裂。
6、能進(jìn)行自我增殖和遺傳
7、新陳代謝
8、細(xì)胞都具有運(yùn)動(dòng)性，包括細(xì)胞自身的運(yùn)動(dòng)和細(xì)胞內(nèi)部的物質(zhì)運(yùn)動(dòng)

相關(guān)產(chǎn)品：

人非小細(xì)胞肺癌細(xì)胞，NCI-H358
人非小細(xì)胞肺癌細(xì)胞，NCI-H524
人非小細(xì)胞肺癌細(xì)胞，NCI-H838
人非小細(xì)胞肺癌細(xì)胞，NCI-H1299
人非小細(xì)胞肺腺癌細(xì)胞，NCI-H1975
人非小細(xì)胞肺腺癌細(xì)胞，NCI-H2087
人非小細(xì)胞肺癌細(xì)胞，HCC827
人大細(xì)胞肺癌細(xì)胞，NCI-H460
人大細(xì)胞肺癌細(xì)胞，NCI-H661
人肺癌細(xì)胞，A549
人肺癌細(xì)胞，H1299
人肺癌細(xì)胞，TKB-1

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