產(chǎn)品名稱：Anti-MSL3L1抗體,MSL3L1蛋白抗體
產(chǎn)品編號(hào)：BYK-17858R
英文名稱：Anti-MSL3L1
中文名稱：MSL3L1蛋白抗體
英文別名：
MSL3;
AU018931;
DKFZp586J1822;
Drosophila MSL3 like 1;
Male specific lethal 3 (Drosophila) like 1;
Male specific lethal 3 homolog (Drosophila);
Male specific lethal 3 homolog 1 (Drosophila);
Male specific lethal 3 homolog 1;
Male specific lethal 3 homolog;
Male specific lethal 3 like 1 (Drosophila);
Male specific lethal 3 like 1;
Male specific lethal 3 protein like 1;
Male-specific lethal 3 homolog;
Male-specific lethal-3 homolog 1;
Male-specific lethal-3 protein-like 1;
MS3L1_HUMAN;
MSL3;
MSL3 like 1;
MSL3-like 1;
Msl31;
MSL3L1;
OTTHUMP00000022911;
OTTHUMP00000022912.
英文介紹：This gene encodes a nuclear protein that is similar to the product of the Drosophila male-specific lethal-3 gene. The Drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus, the human protein is thought to play a similar function in chromatin remodeling and transcriptional regulation, and it has been found as part of a complex that is responsible for histone H4 lysine-16 acetylation. This gene can undergo X inactivation. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 7 and 8. [provided by RefSeq, Jul 2010]
產(chǎn)品規(guī)格：0.1ml/0.2ml
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來電索取說明）
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-MSL3L1抗體,MSL3L1蛋白抗體免疫組化操作流程
1 脫蠟和水化：脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復(fù)：用于福爾馬林固定的石蠟包埋組織芯片：
A 抗原熱修復(fù)
a 高壓熱修復(fù) 在沸水中加入EDTA（ph8.0）或0.01m*緩沖溶液（ph6.0）.蓋上不銹鋼鍋蓋，但不能鎖定。將玻片置于金屬染色加上，緩慢加壓，是玻片在緩沖液中浸泡五分鐘，然后將蓋子鎖定，小閥門將會(huì)升起來。10分鐘后除去熱源，置入涼水中，當(dāng)小閥門沉下去后打開蓋子。此方法適用于較難檢測(cè)或核抗原的抗原修復(fù)。
b 沸熱修復(fù) 電爐或水浴鍋加熱0.01*緩沖液（ph6.0）至95℃左右，放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液（ph6.0）至沸騰后將組織芯片放入，斷電，間隔5-10分鐘，反復(fù)1-2次。適用的抗原：
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法
常用0.1%*和0.4%*液。*使用前預(yù)熱37℃，消化時(shí)間約為5-30分鐘。適用于被固定遮蔽的抗原：包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等
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