產(chǎn)品名稱: Anti-LRRN4CL抗體,富含*重復(fù)蛋白4C端樣蛋白抗體*
產(chǎn)品編號(hào):BYK-18420R
英文名稱:Anti-LRRN4CL
中文名稱:富含*重復(fù)蛋白4C端樣蛋白抗體
英文別名:LRN4L_HUMAN;
LRRN4 C terminal like;
LRRN4 C terminal like protein;
LRRN4 C-terminal-like protein;
Lrrn4cl;
MGC61707.
英文介紹:Leucine-rich repeats (LRRs) are 20-30 amino acid motifs that mediate protein-protein interactions. The primary function of these motifs is to provide a versatile structural framework for the formation of these protein-protein interactions. LRRs are present in a variety of proteins with diverse structure and function, including innate immunity and nervous system development. Several human diseases are associated with mutations in the genes encoding LRR-containing proteins. LRRN4CL (Leucine-rich repeat neuronal protein 4 C-terminal-like protein) is a 238 amino acid single-pass type I membrane protein that contains one fibronectin type-III domain. The gene encoding LRRN4CL maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.
產(chǎn)品規(guī)格:0.1ml/0.2ml
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來電索取說明)
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-LRRN4CL抗體,富含*重復(fù)蛋白4C端樣蛋白抗體* 免疫組化染色:
SP法
(1) 脫蠟、水化 (2)PBS洗2-3次各5分鐘 (3)3%H2O2(80%甲醇)滴加在
TMA上,室溫靜置10分鐘 (4)PBS洗2-3次各5分鐘 (5)抗原修復(fù)
(6)PBS洗2-3次各5分鐘 (7)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(8)滴加Ι抗50μl,室溫靜置1小時(shí)或4℃過夜或37℃1小時(shí)(9)4℃過夜后需在37℃復(fù)溫45分鐘(10)PBS洗3次每次2分鐘
(11)滴加Ⅱ抗45-50μl,室溫靜置或37℃1小時(shí)(12)Ⅱ抗中可加入0.05%的
tween-20.(13)PBS洗3次各5分鐘(14)DAB顯色5-10分鐘,在顯微鏡下掌握染色程度 (15)PBS或自來水沖洗10分鐘 (16)蘇木精復(fù)染2分鐘,鹽酸酒精分化(17)自來水沖洗10-15分鐘 (18)脫水、透明、封片、鏡檢。
SABC法
(1) 脫蠟、水化 (2)PBS洗2次各5分鐘 (3)用蒸餾水或PBS配制新鮮的3%H2O2,室溫封閉5-10分鐘,用蒸餾水洗3次(4)抗原修復(fù)
(5) PBS洗5分鐘 (6)滴加正常山羊血清封閉液,室溫20分鐘,甩去多余液體(7)滴加Ι抗50μl,室溫靜置1小時(shí)或4℃過夜或37℃1小時(shí)
(8)PBS洗3次各2分鐘(9)滴加*化Ⅱ抗,20℃-37℃ 20分鐘
(10)PBS洗3次各2分鐘 (11)滴加試劑SABC 20℃-30℃ 20分鐘
(12) PBS洗4次各5分鐘 (13) DAB顯色:試劑盒或自配顯色劑顯色
(14)脫水、透明、封片、鏡檢。
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