產(chǎn)品名稱：Anti-Gab1抗體,接頭蛋白Gab 1抗體生產(chǎn)廠家

產(chǎn)品編號(hào)：BYK-2770R
英文名稱：Anti-Anti-Gab1
中文名稱：接頭蛋白Gab 1抗體
英文別名：GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform bl; Gab 1; Gab1; GAB1_HUMAN; GRB2 associated binder 1; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1.
英文介紹：Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity (1-3,8). Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), plaet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF.

產(chǎn)品規(guī)格：0.1ml/0.2ml

注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來電索取說明）
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-Gab1抗體,接頭蛋白Gab 1抗體生產(chǎn)廠家免疫組化染色：

一抗孵育(Primary antibody incubation)

參考一抗的說明書，按照適當(dāng)比例用Western一抗稀釋液稀釋一抗。
用微型臺(tái)式真空泵吸盡封閉液，立即加入稀釋好的一抗，室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。如果一抗孵育一小時(shí)效果不佳，可以在4℃緩慢搖動(dòng)孵育過夜?；厥找豢?。加入Western洗滌液，在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液，洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長洗滌時(shí)間并增加洗滌次數(shù)。

二抗孵育(Secondary antibody inucubation)

參考二抗的說明書，按照適當(dāng)比例用Western二抗稀釋液稀釋辣根過氧化物酶(HRP)標(biāo)記的二抗。
用微型臺(tái)式真空泵吸盡洗滌液，立即加入稀釋好的二抗，室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)?；厥斩?，加入Western洗滌液，在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液，洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長洗滌時(shí)間并增加洗滌次數(shù)。

蛋白檢測(cè)(Detection of proteins)

參考相關(guān)說明書，使用BeyoECL, Western 熒光檢測(cè)試劑等ECL類試劑來檢測(cè)蛋白。
洗片時(shí)可以使用X光片自動(dòng)洗片機(jī)。如果沒有自動(dòng)洗片機(jī)，可以用顯影定影試劑盒自行配制顯影液和定影液進(jìn)行手工洗片。

Anti-Gab1抗體,接頭蛋白Gab 1抗體生產(chǎn)廠家其他優(yōu)勢(shì)產(chǎn)品：

byk-0812R    白介素1β/IL-1β抗體    Anti-IL-1 Beta/IL-1B
byk-0555R    白介素1α/IL-1α抗體    Anti-IL-1 Alpha
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byk-2631R    白介素31受體a抗體    Anti-IL-31RA/CRL3
byk-2630R    白細(xì)胞介素31抗體    Anti-IL-31
byk-2629R    白細(xì)胞介素29抗體    Anti-IL-29/IFNL1
byk-2628R    白細(xì)胞介素28B抗體    Anti-IL-28B/IL-28C
byk-2627R    白細(xì)胞介素28A抗體    Anti-IL-28A/IFNL2
byk-2626R    白介素26抗體    Anti-IL-26/AK155
byk-0445R    白介素1受體相關(guān)蛋白樣1前體蛋白抗體    Anti-IL1RAPL1
byk-2625R    白介素22受體結(jié)合蛋白抗體    Anti-IL-22BP/IL22RA2
byk-2624R    白介素-22受體抗體    Anti-IL-22R
byk-2623R    白介素-22抗體    Anti-IL-22
byk-2622R    白介素21受體抗體    Anti-IL-21R
byk-2621R    白介素21抗體    Anti-IL-21
byk-2620R    白介素20受體β鏈抗體    Anti-IL-20RB/IL-20R2
byk-2619R    白介素20受體α鏈抗體    Anti-IL-20R alpha/IL20RA
byk-2618R    白細(xì)胞介素-19抗體    Anti-IL-19/NG.1
byk-1460R    白介素-23受體抗體    Anti-IL-23R 
byk-11460R    表皮生長因子樣蛋白1抗體    Anti-CELSR3/Flamingo homolog 1
byk-11193R    中性鞘磷脂2抗體    Anti-NSMase2
byk-1193R    白介素-23抗體    Anti-IL-23
byk-1834R    白細(xì)胞介素-20抗體    Anti-IL-20
byk-11834R    */*蛋白激酶MNB抗體    Anti-DYRK1A
byk-0529R    白細(xì)胞介素-18/干擾素γ誘導(dǎo)因子抗體    Anti-IL-18/IGIF
byk-2614R    白介素-17F抗體    Anti-MDA7/IL-24/IL-17F
byk-2612R    白介素-17D抗體    Anti-IL-17D/IL-27
byk-2611R    白介素-17C抗體    Anti-IL-17C
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byk-2609R    白介素-17B抗體    Anti-IL-17B
byk-2608R    白介素17受體D抗體    Anti-IL-17RD

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