Anti-Pokemon抗體,撲克蒙蛋白抗體產(chǎn)品現(xiàn)貨銷售,其他同類產(chǎn)品:
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產(chǎn)品名稱:Anti-Pokemon抗體,撲克蒙蛋白抗體
產(chǎn)品編號:BYK-10235R
英文名稱:Anti-Pokemon
中文名稱:撲克蒙蛋白抗體
英文別名:ZBTB7; ZBTB7A; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; FBI1; HIV-1 1st-binding protein 1; Leukemia/lymphoma related factor; LRF; Pokemon; TIP21; TTF-I interacting peptide 21; Zinc finger and BTB domain-containing protein 7A; ZBT7A_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Pokemon抗體,撲克蒙蛋白抗體Western Blotting 具體實驗步驟:
Western,也稱Western blot、Western blotting、Western 印跡,是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進(jìn)行操作:
收集蛋白樣品(Protein sample preparation)
可以使用適當(dāng)?shù)牧呀庖毫呀赓N壁細(xì)胞、懸浮細(xì)胞或組織樣品。對于某些特定的亞細(xì)胞組份蛋白,例如細(xì)胞核蛋白、細(xì)胞漿蛋白、線粒體蛋白等,可以參考相關(guān)文獻(xiàn)提取這些亞細(xì)胞組份蛋白,也可以使用試劑盒進(jìn)行抽提。
收集完蛋白樣品后,為確保每個蛋白樣品的上樣量*,需要測定每個蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同,需要采用適當(dāng)?shù)牡鞍诐舛葴y定方法。因為不同的蛋白濃度測定方法對于一些去垢劑和還原劑等的兼容性差別很大。
Anti-Pokemon抗體,撲克蒙蛋白抗體
英文介紹:Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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