產(chǎn)品名稱:Anti-MDR1抗體,多藥耐藥蛋白/P-糖蛋白抗體
產(chǎn)品編號:BYK-0563R
英文名稱:Anti-MDR1
中文名稱:多藥耐藥蛋白/P-糖蛋白抗體
英文別名:P-Glycoprotein; Multi Drug Resistance Associated Protein, ABCB1; ATP-binding cassette sub-family B (MDR/TAP), member 1; ABC20; CD243; CD243 antigen; CLCS; Multidrug resistance protein 1; P glycoprotein 1; p-GP; gp170; P gp; PGY1; MDR1_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-MDR1抗體,多藥耐藥蛋白/P-糖蛋白抗體
英文介紹:malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-MDR1抗體,多藥耐藥蛋白/P-糖蛋白抗體轉(zhuǎn)膜(Transfer)
我們*在Western實驗中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜(二氟化樹脂膜膜孔徑:0.45um)也可以使用,但硝酸纖維素膜比較脆,在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標(biāo)準(zhǔn)濕式轉(zhuǎn)膜裝置,可以設(shè)定轉(zhuǎn)膜電壓120V,轉(zhuǎn)膜時間為30-60分鐘。具體的轉(zhuǎn)膜時間要根據(jù)目的蛋白的大小而定,目的蛋白的分子量越大,需要的轉(zhuǎn)膜時間越長,目的蛋白的分子量越小,需要的轉(zhuǎn)膜時間越短。
在轉(zhuǎn)膜過程中,特別是高電流快速轉(zhuǎn)膜時,通常會有非常嚴(yán)重的發(fā)熱現(xiàn)象,把轉(zhuǎn)膜槽放置在冰浴中進(jìn)行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn),通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對膜進(jìn)行染色,以觀察實際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍(lán)快速染色液對完成轉(zhuǎn)膜的SDS-PAGE膠進(jìn)行染色,以觀察蛋白的殘留情況。
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