產(chǎn)品名稱：Anti-ACOT8抗體,酰基輔酶A硫酯酶8

產(chǎn)品編號(hào)：BYK-5031R
英文名稱：Anti-ACOT8
中文名稱：?；?硫酯酶8
英文別名：ACTEIII; Acot8; ACOT8_HUMAN; acyl CoA thioesterase 8; Acyl-CoA thioesterase 8; Acyl-coenzyme A thioesterase 8; Choloyl coenzyme A thioesterase; Choloyl-coenzyme A thioesterase; hACTE III; hACTE-III; hACTEIII; HIV Nef associated acyl CoA thioesterase; HIV-Nef-associated acyl-CoA thioesterase; HNAACTE; hTE; Long chain fatty acyl CoA hydrolase; Palmitoyl CoA hydrolase; Peroxisomal acyl CoA thioesterase 1; Peroxisomal acyl coenzyme A thioester hydrolase 1; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long chain acyl CoA thioesterase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE 1; PTE 2; PTE-1; PTE-2; PTE1; PTE2; Thioesterase II.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-ACOT8抗體,?；o酶A硫酯酶8

英文介紹：Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A Thioesterase 8, also known as ACOT8 may mediate Nef-induced down-regulation of CD4. It is a major thioesterase in peroxisomes and competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). It shows a preference for medium-length fatty acyl-CoAs and may be involved in the metabolic regulation of peroxisome proliferation.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Anti-ACOT8抗體,酰基輔酶A硫酯酶8上樣與電泳:

冷卻到室溫后，把蛋白樣品直接上樣到SDS-PAGE膠加樣孔內(nèi)即可。為了便于觀察電泳效果和轉(zhuǎn)膜效果，以及判斷蛋白分子量大小，使用預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)。
電泳時(shí)通常*在上層膠時(shí)使用低電壓恒壓電泳，而在溴酚藍(lán)進(jìn)入下層膠時(shí)使用高電壓恒壓電泳。對(duì)于Bio-Rad的標(biāo)準(zhǔn)電泳裝置或類似電泳裝置，低電壓可以設(shè)置在80-100V，高電壓可以設(shè)置在120V左右。SDS-PAGE可以采用普通的電泳儀就可以滿足要求。為了電泳方便起見，也可以采用整個(gè)SDS-PAGE過程恒壓的方式，通常把電壓設(shè)置在100V，然后設(shè)定定時(shí)時(shí)間為90－120分鐘。設(shè)置定時(shí)可以避免經(jīng)常發(fā)生的電泳過頭。
通常電泳時(shí)溴酚藍(lán)到達(dá)膠的底端處附近即可停止電泳，或者可以根據(jù)預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)的電泳情況，預(yù)計(jì)目的蛋白已經(jīng)被適當(dāng)分離后即可停止電泳。

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