產(chǎn)品名稱：Anti-Cenexin1抗體,精尾部結(jié)構(gòu)蛋白/ODF2抗體

產(chǎn)品編號(hào)：BYK-4147R
英文名稱：Anti-Cenexin1
中文名稱：精尾部結(jié)構(gòu)蛋白/ODF2抗體
英文別名：ODF2; sperm tail structural protein; 84 kDa outer dense fiber protein; Cenexin 1; Cenexin1 variant 1; KKT 4; KKT4; ODF 2; ODF 84; ODF2; ODF2/1; ODF2/2; ODF84; Outer dense fiber of sperm tail 2; Outer dense fiber of sperm tails 2; outer dense fiber of sperm tails; Outer dense fiber of sperm tails protein 2; Outer dense fiber of sperm tails, 84 kD; Outer dense fiber protein 2; Sperm outer dense fiber major protein 2; Testis specific autoantigen; ODFP2_HUMAN.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-Cenexin1抗體,精尾部結(jié)構(gòu)蛋白/ODF2抗體

英文介紹：Cenexin1 is an isoform of ODF2, that unlike ODF2 is present in several somatic cell types. Cenexin1 acts as a general scaffold protein that is specifically localised to the distal/subdistal appendages of mother centrioles. Cenexin1 is required for proper localization of Plk1 to the centrosomes. This centrosomal localization of Plk1 is required for proper microtubule function. Cenexin1 recruits Plk1 via a C-terminal extension of cenexin1 that is not present in ODF2. Cenexin1 is required for proper mitotic progression; depletion of Cenexin1 ultimay leads to chromosome missegregation and apoptosis. 

The ODF2 (outer dense fiber 2) gene encodes both ODF2 and Cenexin1, which have very different functions. ODF2 is a major component of sperm tail outer dense fibers (ODFs). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail. They may help to maintain the passive elastic structures and elastic recoil of the sperm tail, and may also modulate sperm motility.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Anti-Cenexin1抗體,精尾部結(jié)構(gòu)蛋白/ODF2抗體轉(zhuǎn)膜(Transfer)

我們*在Western實(shí)驗(yàn)中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜（二氟化樹脂膜膜孔徑：0.45um)也可以使用，但硝酸纖維素膜比較脆，在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請(qǐng)參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標(biāo)準(zhǔn)濕式轉(zhuǎn)膜裝置，可以設(shè)定轉(zhuǎn)膜電壓120V，轉(zhuǎn)膜時(shí)間為30-60分鐘。具體的轉(zhuǎn)膜時(shí)間要根據(jù)目的蛋白的大小而定，目的蛋白的分子量越大，需要的轉(zhuǎn)膜時(shí)間越長，目的蛋白的分子量越小，需要的轉(zhuǎn)膜時(shí)間越短。
在轉(zhuǎn)膜過程中，特別是高電流快速轉(zhuǎn)膜時(shí)，通常會(huì)有非常嚴(yán)重的發(fā)熱現(xiàn)象，把轉(zhuǎn)膜槽放置在冰浴中進(jìn)行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)，通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對(duì)膜進(jìn)行染色，以觀察實(shí)際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍(lán)快速染色液對(duì)完成轉(zhuǎn)膜的SDS-PAGE膠進(jìn)行染色，以觀察蛋白的殘留情況。

Anti-Cenexin1抗體,精尾部結(jié)構(gòu)蛋白/ODF2抗體產(chǎn)品現(xiàn)貨銷售，其他同類產(chǎn)品：

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