產(chǎn)品名稱:Anti-PLAC8抗體,胎盤特異基因8蛋白抗體
產(chǎn)品編號:BYK-15584R
英文名稱:Anti-PLAC8
中文名稱:胎盤特異基因8蛋白抗體
英文別名:C15; Onzin; PLAC8; PLAC8_HUMAN; Placenta specific 8; Placenta-specific gene 8 protein; Protein C15.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-PLAC8抗體,胎盤特異基因8蛋白抗體
英文介紹:PLAC8 is a 115 amino acid protein belonging to the cornifelin family that is expressed at high levels in plasmacytoid dendritic cells and other organs of the immune system including lymph nodes, spleen, bone marrow and peripheral blood leukocytes, with lower expression in appendix, thymus and fetal liver. Human PLAC8 shares 83% homology with murine PLAC8, where it has been identified in placenta from 9.5-18.5 days postcoitum. The gene encoding PLAC8 maps to human chromosome 4, which represents approximay 6% of the human genome and contains nearly 900 genes. Notably, the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease, is on chromosome 4. FGFR-3 is also encoded on chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease
此產(chǎn)品本公司有標記的一抗出售,相關(guān)標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-PLAC8抗體,胎盤特異基因8蛋白抗體Western Blotting 具體實驗步驟:
Western,也稱Western blot、Western blotting、Western 印跡,是用抗體檢測蛋白的重要方法之一。
Western可以參考如下步驟進行操作:
收集蛋白樣品(Protein sample preparation)
可以使用適當?shù)牧呀庖毫呀赓N壁細胞、懸浮細胞或組織樣品。對于某些特定的亞細胞組份蛋白,例如細胞核蛋白、細胞漿蛋白、線粒體蛋白等,可以參考相關(guān)文獻提取這些亞細胞組份蛋白,也可以使用試劑盒進行抽提。
收集完蛋白樣品后,為確保每個蛋白樣品的上樣量*,需要測定每個蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同,需要采用適當?shù)牡鞍诐舛葴y定方法。因為不同的蛋白濃度測定方法對于一些去垢劑和還原劑等的兼容性差別很大。
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