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產(chǎn)品名稱:Anti-RAB5抗體,ras癌基因家族Rab5蛋白抗體
產(chǎn)品編號:BYK-6175R
英文名稱:Anti-RAB5
中文名稱:ras癌基因家族Rab5蛋白抗體
英文別名:RAB 5; RAB 5A; RAB5A; RAB5A member RAS oncogene family; RAB5A_HUMAN; RAS associated protein RAB5A; Ras related protein Rab 5A; Ras-related protein Rab-5A.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-RAB5抗體,ras癌基因家族Rab5蛋白抗體
英文介紹:Rab5-related subfamily. This subfamily includes Rab5 and Rab22 of mammals, Ypt51/Ypt52/Ypt53 of yeast, and RabF of plants. The members of this subfamily are involved in endocytosis and endocytic-sorting pathways. In mammals, Rab5 GTPases localize to early endosomes and regulate fusion of clathrin-coated vesicles to early endosomes and fusion between early endosomes. In yeast, Ypt51p family members similarly regulate membrane trafficking through prevacuolar compartments. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-RAB5抗體,ras癌基因家族Rab5蛋白抗體電泳(Electrophoresis):
(1)、SDS-PAGE凝膠配制
SDS-PAGE凝膠可以參考一些文獻(xiàn)資料進(jìn)行配制,也可以使用博研生物生產(chǎn)的SDS-PAGE凝膠配制試劑盒。該試劑盒提供了除水和配膠器具外的所有試劑以及配制各種濃度SDS-PAGE的配方。
(2)、樣品處理
在收集的蛋白樣品中加入適量濃縮的SDS-PAGE蛋白上樣緩沖液。例如2X或5X的SDS-PAGE蛋白上樣緩沖液。使用5X的SDS-PAGE蛋白上樣緩沖液可以減小上樣體積,在相同體積的上樣孔內(nèi)可以上樣更多的蛋白樣品。5X的SDS-PAGE蛋白上樣緩沖液可以參考相關(guān)文獻(xiàn)資料配制,也可以使用我公司生產(chǎn)的SDS-PAGE蛋白上樣緩沖液(5X) 。100℃或沸水浴加熱3-5分鐘,以充分變性蛋白。
(3)、上樣與電泳
冷卻到室溫后,把蛋白樣品直接上樣到SDS-PAGE膠加樣孔內(nèi)即可。為了便于觀察電泳效果和轉(zhuǎn)膜效果,以及判斷蛋白分子量大小,使用預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)。
電泳時通常*在上層膠時使用低電壓恒壓電泳,而在溴酚藍(lán)進(jìn)入下層膠時使用高電壓恒壓電泳。對于Bio-Rad的標(biāo)準(zhǔn)電泳裝置或類似電泳裝置,低電壓可以設(shè)置在80-100V,高電壓可以設(shè)置在120V左右。SDS-PAGE可以采用普通的電泳儀就可以滿足要求。為了電泳方便起見,也可以采用整個SDS-PAGE過程恒壓的方式,通常把電壓設(shè)置在100V,然后設(shè)定定時時間為90-120分鐘。設(shè)置定時可以避免經(jīng)常發(fā)生的電泳過頭。
通常電泳時溴酚藍(lán)到達(dá)膠的底端處附近即可停止電泳,或者可以根據(jù)預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)的電泳情況,預(yù)計目的蛋白已經(jīng)被適當(dāng)分離后即可停止電泳。
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