Thermo Scientific Remel 腸球菌/A 族鏈球菌篩選試劑盒用于通過七葉苷水解和 PYR 反應(yīng)對腸球菌和 A 族鏈球菌進行推斷性鑒定。
詳細介紹
Presumptively identify Group A Streptococci and Enterococci using Enterococcus/Group A Strep Screen.
Easy to store - No special storage required
Easy to interpret - With visual inspection - the color change in the medium to dark brown indicates a positive esculin reaction and after addition of two drops of Cinnamaldehyde Reagent to the tube, the color changes to red indicating a positive PYR test.
This medium contains esculin compound which is hydrolyzed enzymatically at the β-glucose linkage to yield two products – esculetin and glucose. Esculetin reacts with the ferric ammonium citrate present in the medium to form a phenolic iron complex which is brown-black in color.
L-pyroglutamic acid β-naphthylamide (PYR) serves as the substrate for the detection of pyrrolidonyl peptidase. Following substrate hydrolysis by the peptidase enzyme, the resulting β-naphthylamine produces a red color upon the addition of ρ-dimethyl-aminocinnamaldehyde (Cinnamaldehyde Reagent).
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