上海研盟生物科技有限公司Anti-Kv1.8抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-Kv1.8 antibody
中文名稱:電壓門控鉀通道Kv1.8抗體
電壓門控鉀通道Kv1.8抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
電壓門控鉀通道Kv1.8抗體,Anti-Kv1.8抗體抗原抗體反應:
一、抗原抗體反應特點
1.高度特異性,特異性是抗原抗體反應的zui主要特征,特定抗原決定簇只與特定抗體結(jié)合;
2.可逆性:抗原與抗體結(jié)合形成抗原抗體復合物的過程是一種動態(tài)平衡, 其反應式為:Ag+AbAg·Ab;
3.適當濃度和比例;
4.敏感性:在測定血清中某一物質(zhì)的含量時,化學比色法的敏感度為mg/ml水平,酶反應測定法的敏感度約為5~10μg/ml,免疫測定中凝膠擴散法的敏感度與酶反應法相仿。
二、影響抗原抗體反應的因素
1.電解質(zhì) :抗原抗體特異結(jié)合后,膠體粒便可發(fā)生脫水作用,此時易受電解質(zhì)影響,如有適當濃度的電解質(zhì)存在就會使它們失去部分負電荷而相互凝聚,于是出現(xiàn)明顯的凝集或沉淀現(xiàn)象。
2.酸堿度:合適的pH為6-8。
3.溫度:37℃通常是抗原體反應zui適溫度。
★研盟生物★電壓門控鉀通道Kv1.8抗體,Anti-Kv1.8抗體
免疫染色:
下面以石蠟切片的免疫組化為例演示詳細操作流程(SP法)
1.60℃烤片2小時
2.脫蠟。二甲苯脫蠟15分鐘,三次
3.水化。依次經(jīng)過無水乙醇、95%、90%、80%、70%,各5分鐘,蒸餾水(或自來水)5分鐘
4.PBS(洗液C-0079)洗5分鐘,三次
5.抗原修復。枸櫞酸緩沖液(修復液,C-0070)(約92-95℃)煮沸(沸水?。┬迯?5分鐘,自然涼至室溫。PBS洗5分鐘,三次
6.3%雙氧水(包含在SP-0023中)消化內(nèi)源性的過氧化物酶,20分鐘,PBS洗5分鐘,三次
7.正常山羊血清(包含在SP-0023中)封閉,37℃20分鐘
8.一抗孵育,4℃過夜. (抗體稀釋液C-0007)
9.PBS洗5分鐘,三次
10.二抗孵育,37℃20分鐘(二抗為*標記的羊抗兔IgG,包含在SP-0023中),PBS洗5分鐘,三次
11.三抗孵育,37℃20分鐘(三抗為辣根酶標記的鏈霉親和素,包含在SP-0023中),PBS洗5分鐘,三次
12.DAB(顯色劑C-0010)顯色,鏡下觀察結(jié)果,適時終止
13.蘇木素(C-0021)復染5分鐘,自來水沖洗片刻,1%的鹽酸酒精(75%)溶液(此試劑無法銷售,請自行配置,配制比例為:100ml 75%酒精+1ml濃鹽酸)分化30秒,自來水沖洗5分鐘藍化
14.脫水。依次經(jīng)過70%、80%、90%、95%、無水乙醇,各5分鐘,二甲苯15分鐘,三次
15.封片,中性樹膠(C-0073)封片
★研盟生物 ★合作: ★客服
相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
簡單介紹:
產(chǎn)品別名:Cyclic GMP gated potassium channel; KCA10_HUMAN; Kcn 1; Kcn1; KCNA 10; KCNA10; Kv1.8; Potassium voltage gated channel shaker related subfamily member 10; Potassium voltage gated channel subfamily A member 10; Potassium voltage-gated channel subfamily A member 10; Voltage gated potassium channel subunit Kv1.8; Voltage-gated potassium channel subunit Kv1.8.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It is specifically regulated by cGMP and postulated to mediate the effects of substances that increase intracellular cGMP. This gene is intronless, and the gene is clustered with genes KCNA2 and KCNA3 on chromosome 1.
相關優(yōu)質(zhì)產(chǎn)品:
BLAME/SLAMF8(B Lymphocyte Activator Macrophage-expressed) BLAME抗體
Cdc2/P34CDC2/CDK1(Cell division control protein 2) 周期素依賴激酶1抗體
CD335/NKp46/NCR1 細胞毒性受體NK-p46抗體
CD336/NKp44/NCR2(Natural killer cell p44-related protein) 細胞毒性受體NK-p44抗體
CD337/NKp30/NCR3(Natural killer cell p30-related protein) 細胞毒性受體NK-p30抗體
CD328/Siglec-7(Sialic acid-binding Ig-like lectin 7) 唾液酸結(jié)合性免疫球蛋白樣凝集素7抗體
KLRF1/NKp80 細胞毒性受體NK-p80抗體
Cdc6 細胞分裂周期蛋白6抗體
CD339/Jagged1 抗Jagged1/CD339抗體
Cdc34(Cell Division Cycle protein 34) 細胞周期調(diào)控因子34
CDK1 (Cyclin-Dependent Kinase 1) 周期素依賴性激酶1抗體
CDK2 (Cyclin-Dependent Kinase 2) 周期素依賴性激酶2抗體
CDK3 (cyclin-dependent kinase 3) 周期素依賴性激酶3抗體
CDK4 (cyclin-dependent kinase 4) 周期素依賴性激酶4抗體
CDK5(Cyclin Dependent Kinase 5) 周期素依賴性激酶5抗體
CDK6 (Cyclin Dependent Kinase 6) 周期素依賴性激酶6抗體
CDK7/Cyclin H/MAT1 周期素依賴性激酶7抗體
CDK8(Cyclin Dependent Kinase 8) 周期素依賴性激酶8抗體
CCNT1/CDK9 (Cyclin Dependent Kinase 9) 周期素依賴性激酶9抗體