★研盟生物 ★合作: ★客服
相關(guān)標(biāo)記抗體:HRP標(biāo)記抗體,Biotin標(biāo)記抗體,Gold標(biāo)記抗體,RBITC標(biāo)記抗體,AP標(biāo)記抗體,FITC標(biāo)記抗體,Cy3標(biāo)記抗體,Cy5標(biāo)記抗體,Cy5.5標(biāo)記抗體,Cy7標(biāo)記抗體,PE標(biāo)記抗體,PE-Cy3標(biāo)記抗體,PE-Cy5標(biāo)記抗體,PE-Cy5.5標(biāo)記抗體,PE-Cy7標(biāo)記抗體,APC標(biāo)記抗體,Alexa Fluor 350標(biāo)記抗體,Alexa Fluor 488標(biāo)記抗體,Alexa Fluor 555標(biāo)記抗體,Alexa Fluor 647標(biāo)記抗體
上海研盟生物科技有限公司Anti-Perforin抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱(chēng):Anti-Perforin antibody
中文名稱(chēng):穿孔素抗體
穿孔素抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
★研盟生物★穿孔素抗體,Anti-Perforin抗體
免疫組化操作步驟
(一)、儀器設(shè)備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風(fēng)裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標(biāo)本;pH7.0-7.4適合光學(xué)纖維標(biāo)本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無(wú)水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片
穿孔素抗體,Anti-Perforin抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了。抗體溶解后,置于-20℃保存,分裝后使用,避免反復(fù)凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補(bǔ)足,置于-20℃保存,這樣抗體不會(huì)凍,有利于抗體的穩(wěn)定。
后續(xù)試驗(yàn)時(shí),再使用對(duì)應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個(gè)月有效;若在-20℃下保存(*),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
簡(jiǎn)單介紹:
產(chǎn)品別名:Cytolysin; FLH2; HPLH2; Lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; PERF_HUMAN; PERF_MOUSE; Perforin 1; Perforin 1 precursor; Perforin 1 preforming protein; Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF; Pore forming protein; PRF 1; PRF1; SHGC-10760.
抗體來(lái)源:Rabbit or Mouse
保質(zhì)期:1年
克隆類(lèi)型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.
相關(guān)優(yōu)質(zhì)產(chǎn)品:
p-PKC(phospho-Protein Kinase C) 磷酸化蛋白激酶C抗體
PLAU/uPA (Plasminogen activator,urokinase) *型纖溶酶原激活因子抗體
PLAUR/uPAR(Plasminogen activator,urokinase receptor) *型纖溶酶原激活因子受體抗體
PLG(Plasminogen) 纖維蛋白溶酶原抗體
PLGF (Placenta growth factor human) 胎盤(pán)生長(zhǎng)因子(抗體)相關(guān)抗體 bs-0280R(抗大鼠) bs-0234R(抗小鼠)
PLGF (Placenta growth factor mouse) 小鼠胎盤(pán)生長(zhǎng)因子抗體
PLGF(Placenta growth factor) 胎盤(pán)生長(zhǎng)因子抗體
PMP-22(Peripheral Myelin Protein) 外周髓鞘蛋白-22抗體
PMS2(Post Meiotic Segregation Increased 2) 腫瘤錯(cuò)配修復(fù)基因PMS2抗體
Podoplanin Protein 平足蛋白抗體
Pokemon/ZBTB7A “撲ke蒙”蛋白又稱(chēng)“波克曼”蛋白抗體
Polycystin 1(Autosomal dominant polycystic kidney disease protein 1) 多囊腎蛋白1抗體
Polycystin 2 多囊腎蛋白2抗體
PP-1 蛋白磷酸酯酶-1抗體
PP-2A (protein phosphatase 2A) 蛋白質(zhì)磷酸酶-2A抗體
PP-2A(protein phosphatase 2A catalytic subunit) 蛋白質(zhì)磷酸酶-2A抗體
PP2Ac 磷酸酯酶-2Ac抗體
PPAR-delta (peroxisome proliferator-activated receptor;PPAR-beta) D型-過(guò)氧化酶活化增生受體抗體