變綠粘球菌培養(yǎng)
低溫存法:
①簡單保存法,將瓊脂斜面孢子培養(yǎng)物、菌絲懸浮液以及由麩皮、大米、小米等谷物原料制成的孢子培養(yǎng)物置于4℃冰箱保存,保存時間不超過1~2個月,若將谷物原料制備的孢子瓶抽真空并在棉塞上浸蠟,以隔絕外界空氣和汽,保持時間可達(dá)3~4個月;②液氮超低溫保存法,將生長穩(wěn)定期的細(xì)胞懸浮在10%甘油或其他低冰點(diǎn)液體中,密封于安瓿管內(nèi),然后控制冷卻速度,使安瓿管溫度逐步下降至 -35℃時,即可置于-150~-196℃的液氮罐中保存。大多數(shù)微生物如病毒、噬菌體、多種細(xì)菌、放線菌、酵母和原蟲、特別是一些用冷凍干燥法有困難的微生物,都可用此法*保存。
產(chǎn)品名稱: 變綠粘球菌培養(yǎng)
英文名稱:Myxococcus virescens
型號:CS-JZ0009培養(yǎng)保藏法:培養(yǎng)后于4—6℃冰箱內(nèi)保存。
庫存:提供現(xiàn)貨
保存:低溫避光保存
注意事項:
l、微生物菌種應(yīng)保藏于低溫、清潔和干燥的地方,室溫放置時問過長會導(dǎo)致菌種衰退;
2、菌種操作應(yīng)在無菌條件下進(jìn)行,防止雜菌污染:
3、斜面菌種保藏時間通常為1-2個月,應(yīng)根據(jù)菌種狀況及時轉(zhuǎn)接;凍干菌種保藏時間通常為5~10年;
4、菌種使用過程中如出現(xiàn)雜菌污染或菌種生產(chǎn)性能下降,應(yīng)及時與我公司或更換新的菌種。
作用機(jī)理及使用效果
1、耗氧產(chǎn)酸,調(diào)節(jié)腸道微生態(tài)平衡,達(dá)到預(yù)防腹瀉、下痢等疾病
枯草芽孢桿菌在生長過程中耗氧,造成腸道低氧,并產(chǎn)生丙酸、丁酸等有機(jī)酸降低腸道內(nèi)PH值,促進(jìn)有益厭氧菌如乳酸菌、雙歧桿菌等的生長,同時抑制腸道病原細(xì)菌如大腸桿菌、沙門氏菌等的生長繁殖,提高有益微生物在腸道細(xì)菌種間的競爭優(yōu)勢,達(dá)到預(yù)防豬腹瀉及下痢的目的。
2、分泌多種酶類,提高飼料消化利用率
枯草芽孢桿菌可分泌多種酶如蛋白酶、*、脂肪酶、果膠酶、葡聚糖酶等,降解動植物性飼料中復(fù)雜有機(jī)物,促進(jìn)消化吸收,提高飼料利用率。
3、產(chǎn)生多種營養(yǎng)素,參與機(jī)體新陳代謝
枯草芽孢桿菌在動物腸道內(nèi)生長繁殖,能產(chǎn)生維生素、促生長因子和蛋白多肽類抗菌物質(zhì)等多種營養(yǎng)物質(zhì),還可以提高動物對鈣、磷的利用率,參與機(jī)體的新陳代謝。減少糞便中氮、磷、鈣的排泄量,減少有害氣體排放,降低養(yǎng)殖場所氨氣、吲哚等有害氣體的濃度,減輕糞便臭味,改善養(yǎng)殖環(huán)境。
4、提高動物免疫力
枯草芽孢桿菌能夠刺激動物免疫器官的生長發(fā)育,激活淋巴細(xì)胞,提高免疫球蛋白和抗體水平,通過淋巴循環(huán)活化全身的免疫防御系統(tǒng),提高動物免疫力和抗病力。同時緩解厭食,生長緩慢等應(yīng)激反應(yīng)。
人生長激素釋放肽(Ghrelin)96T/48TAnti-CT
人*過氧化物酶(GSH-PX)96T/48TAnti-CT DNA
人同型半胱氨(HCY)96T/48TAnti-CTGF
人脂多糖結(jié)合蛋白(LBP)96T/48TAnti-CTLA-4/CD152
人脂多糖(LPS)96T/48Tanti-CTn1
人髓樣分化蛋白-2(MD2)96T/48TAnti-CT-R
人α1微球蛋白(MGα1)96T/48TAnti-CULLIN/Cdc53/CUL/SCFCdc4
英文名稱 Anti-GLS1
中文名稱 *酶1抗體
別 名 mitochondrial; AAD20; GLS; GLS1; GLSK_HUMAN; Glutaminase kidney isoform; K-glutaminase; KIAA0838; L-glutamine amidohydrolase; DKFZp686O15119; FLJ10358.
濃 度 1mg/1ml
規(guī) 格 0.2ml/200μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep
產(chǎn)品類型 一抗
研究領(lǐng)域 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 線粒體
蛋白分子量 predicted molecular weight: 72kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GLS1
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].
Function : Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Subunit : Heterotetramer. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.
Subcellular Location : Isoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion.
Tissue Specificity : Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Similarity : Belongs to the glutaminase family.
Contains 2 ANK repeats.
Database links : UniProtKB/Swiss-Prot: O94925.1
英文名稱 Anti-Phospho-Glycogen synthase 1(Ser640)
中文名稱 磷酸化葡萄糖合成酶1抗體
別 名 Glycogen synthase 1 (phospho S640); Glycogen synthase 1 (phospho Ser640); p-Glycogen synthase 1 (S640); Glycogen synthase 1 (muscle); Glycogen synthase 1; GSY; GYS; GYS1; EC 2.4.1.11; Glycogen synthase1; GYS 1; Starchsynthase muscle; UDP glucose glycogen glucosyltransferase; GYS1_HUMAN; Glycogen [starch] synthase, muscl.
濃 度 1mg/1ml
規(guī) 格 0.1ml/100μg
抗體來源 Rabbit
克隆類型 polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Cat
產(chǎn)品類型 一抗 磷酸化抗體
研究領(lǐng)域 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 糖尿病 新陳代謝
蛋白分子量 predicted molecular weight: 81kDa
性 狀 Lyophilized or Liquid
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Glycogen synthase 1 around the phosphorylation site of Ser640
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復(fù))
not yet tested in other applications.