表皮生長因子, SIGMA E4127-0.1MG
分類 抗體
名稱 sigma抗體表皮生長因子
級別 優(yōu)級純GR
產(chǎn)品規(guī)格 SIGMA E4127-0.1MG
CAS 62229-50-9
品牌 Sigma
用途范圍 生物科研
保存溫度 2-8℃
英文名稱 Epidermal Growth Factor from murine submaxillary g
供應(yīng)商 上海研伴實(shí)業(yè)有限公司
sigma抗體表皮生長因子
表皮生長因子 來源于小鼠頜下腺 品牌：SIGMA
suitable for cell culture
· 產(chǎn)品編號：E4127
· 中文名稱：表皮生長因子 來源于小鼠頜下腺
· 英文名稱：Epidermal Growth Factor from murine submaxillary gland
· 英文別名:EGF Epidermal cell growth factor
· CAS號：62229-50-9
· 保存溫度：2-8 °C
· EC號：263-468-7
· MDL號：MFCD01634807
訂貨信息：
產(chǎn)品規(guī)格價格(RMB)
· E4127-0.1MG1,838.07元
· E4127-5X0.1MG7,376.85元
注：此價格僅供參考，請以SIGMA價格為準(zhǔn)，優(yōu)惠價請電詢。
性能描述
Analysis Note
The biological activity is measured in a proliferation assay using BALB/MK cells.
Biochem/physiol Actions
Qualified for use as a mitogen for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.
Preparation Note
Purified by gel filtration.
Physical form
Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.
General description
Epidermal Growth Factor (EGF) is a 6 kDa polypeptide discovered by Cohen and Levi -Montalcini and isolated from mouse submaxillary glands. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells. In tissue cultures EGF can act to reduce or eliminate the requirement for serum and is often used in conjunction with other media additives and hormones, such as Insulin (Product No. I1882), Transferrin (Product No. T 5391), and Prostaglandin E1 (Product No. P7527). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins.
Human EGF was originally isolated from human urine as β-Urogastrone, an inhibitor of gastric acid secretion. Mouse and human EGF are 70% homologous in their sequence of 53 amino acids, act upon the same EGF receptor, elicit nearly identical biological effects and are highly inter-species cross-reactive.
EGF is structurally homologous to human Transforming Growth Factor-α (Product Nos. T7533 and T2035), which exerts its actions through EGF receptors. EGF is also homologous to a sequence contained in a 19 kDa protein of Vaccinia virus, which appears to utilize the EGF receptor to gain entry into cells. The EGF receptor is a 170 kDa glycoprotein having EGF-activated protein tyrosine kinase activity. Plaet-Derived Growth Factor(PDGF, Product No. P8147) transmodulates the EGF receptor by reduc ing both its EGF affinity and its kinase activity.
性質(zhì)
Related Categories | Cancer Research Cell Biology Cell Culture Cell Culture Supplies Cell Signaling and Neuroscience更多... |
form | lyophilized powder |
potency | 0.1-10 ng/mL ED50/EC50 |
total impurities | endotoxin, tested |
suitability | suitable for cell culture |
storage temp. | 2-8°C |
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