抗體小量純化試劑盒，CY-18110
分類 抗體
級別 超純,高純
含量 100（％）
產品規(guī)格 10次
CAS CY-18110
供應商 上海研伴實業(yè)有限公司
用途范圍 純化抗體
保存溫度 2-8℃
英文名稱 Antibody Purification Mini Kit
特點 填料為原裝GE！
填料為原裝的GE??！國產的價格??！
抗體小量純化試劑盒，來源于*的蛋白A與抗體的不變區(qū)有多個結合位點。將蛋白A與Sepharose共價交聯(lián)制備的層析介質，可以用于抗體純化。抗體與蛋白A在高鹽高pH值條件下結合，在低鹽pH值條件下解離。不同來源的抗體與蛋白A的結合能力有較大差異，結合與解離的條件也有區(qū)別。
訂貨信息
中文名稱 | 英文名稱 | 供應商 | 產品貨號 | 規(guī)格 | 價格（￥） |
抗體小量純化試劑盒 | Antibody Purification Mini Kit | 研伴實業(yè) | CY-18110 | 10T | 電儀 |
Protein A對不同來源IgG結合劑量
| 高親和性（如兔） | 低親和性（如小鼠） |
Protein A Sepharose 4 FF結合劑量 | 35 mg/ml | 3-10 mg/ml |
Protein A Sepharose 4 FF每次用量 | 40微升 | 40微升（50%懸液） |
每次純化抗體數(shù)量 | 700微克 | 60-200微克 |
一般高滴度抗血清中IgG濃度
| 小鼠 | 兔抗血清 | 羊 |
IgG濃度 | 10 mg/ml | 5 mg/ml | 20 mg/ml |
Mini kit
PRE-EQUILIBRATION
1.Equilibrate the Spin Column with0.6mL Binding Buffer A by centrifuging the spin column at 500 × g for1minutes.
NOTE: If using one spin column, ensure that the spin column is counterbalanced with a unit of equal weight (adjusted
with distilled water).
CLARIFICATION OF SAMPLE
2.Pre-filter the sample (e.g., tissue culture supernatant, serum or ascites) through a 0.22 μmSyringeFilter toremoveany debris immediay before loading the sample.Protein precipitation is common during storage and when repeatedfreeze/thaw cycles in ascites, sera and tissue culture supernatants occur. Newly formed aggregates and precipitatingprotein complexes can foul theProteinA media and result in significantly slower flow rates. Ensure that thesamples are filteredimmediaybefore loading, using filters with pore sizes no greater than 0.22 μm. It is critical tothe optimal performance of these devices that these instructions are rigorously followed.
SAMPLE LOADING
3.Dilute the filtered sample 1:1 v/v in Binding Buffer A. (For example, add 1 mL filtered sample to 1 mL buffer.) Pipettethe0.6mL sample into the spin column. Centrifuge the spin column at 100–150 × g for1minutes.Ideal
sample loadingconditions are obtained using a flow rate of less than 1 mL/min. It may be necessary to increase the spin time or spinspeed if any sample remainsincolumn. Alternatively, a flow rate slower than expected is indicative
of a partiallyclogged plug resulting from incomplete filtration of the sample.
WASHING
4.Wash the spin column to remove unbound contaminants by adding0.6mL Binding Buffer A and centrifuging the spincolumn for1minutes at 500 × g. Add another0.6mL Binding Buffer A and centrifuge for2more minutes at 500 × g.Theunbound wash will contain non-immunoglobulin components. A flow rate slower than expected is indicative of apartially clogged plug resulting from incomplete filtration of the sample. In the unlikely event that flow rates are
significantly slower than those expected, increase the centrifugal speed to 1000 × g with2minute spin time bursts.
ELUTION
■For purifying mouse IgG1, rat IgG1, rat IgG2a, rat IgG2b and bovine IgG1 (or if you are unsure which IgG subclass youare purifying), use both elution steps 5 and 6 for your initial kit use in order to establish the mildest elution conditionpossible for the antibody. Analyze the two fractions in separate tubes to avoid sample dilution.
■For purifying mouse IgG2a, mouse IgG2b, mouse IgG3, rat IgG2c, human IgG1-IgG4, rabbit IgG, guinea pig IgG1,
guinea pig IgG2, bovine IgG2 and any other IgGs, proceed to elution step 6 only.
■A flow rate slower than expected is indicative of a partially clogged plug resulting from incomplete filtration of the
sample. In the unlikely event that flow rates are significantly slower than those expected, increase the centrifugal speedto 1000 × g with2minute spin time bursts.
5.Elute the bound IgG with0.1mL Elution Buffer B1 directly into a fresh centrifuge tube containing 0.005 mL NeutralizationBuffer C to bring the sample to neutral pH. Centrifuge the spin column for 5 minutes at 500 × g.Save the sample for analysis
6.Elute the bound IgG with0.1mL Elution Buffer B2 directly into a fresh centrifuge tube containing0.013 mL NeutralizationBuffer C to bring the sample to neutral pH. Centrifuge the spin column for 5 minutes at 500 × g.Save the sample for analysis.
REGENERATION
7.Wash the media with0.6mL Elution Buffer B2 by centrifuging the spin column at 500 × g for2minutes.
Re-equilibrate themedia with0.6mL of Binding Buffer A by centrifuging the spin column at 500 × g for 2 minutes.
DESALTING AND CONCENTRATING
8.If necessary, desalt and concentrate the antibody preparation using the Ultra centrifugal filter device with30,000 NMWL. Add 0.05-0.5% w/v sodium azide if the antibodies are to be stored at 2–8 °C.
We recommend freezing the antibodies in small aliquots in 50% glycerol at -20 °C for long term storage.
抗體小量純化試劑盒，CY-18110上海研伴實業(yè)有限公司其他相關產品目錄：
JW.MO1721 | 小鼠類風濕因子IgM(RF IgM)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1722 | 小鼠類*(TPS)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1723 | 小鼠粒細胞集落刺激因子(G-CSF)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1724 | 小鼠粒細胞巨噬細胞集落刺激因子(GM-CSF)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1725 | 小鼠裂解型糖基化終末產物受體(cRAGE)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1726 | 小鼠淋巴毒素α(LTA)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1727 | 小鼠淋巴細胞功能相關抗原1(LFA-1)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1728 | 小鼠淋巴細胞功能相關抗原3(LFA-3/CD58)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1729 | 小鼠淋巴細胞趨化因子(Lptn/LTN/XCL1)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1730 | 小鼠磷酸*(PC)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1731 | 小鼠磷酸二酯酶(PDE)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1732 | 小鼠磷酸二酯酶3B(PDE3B)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1733 | 小鼠磷酸二酯酶4(PDE4)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1734 | 小鼠磷酸甘油酸激酶(PGK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1735 | 小鼠磷酸果糖激酶1(PFK-1)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1736 | 小鼠磷酸化AKT蛋白(p-AKT)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1737 | 小鼠磷酸化p38絲裂原活化蛋白激酶(p-P38MAPK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1738 | 小鼠磷酸化Tau蛋白(p-Tau)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1739 | 小鼠磷酸化蛋白激酶C(P-PKC)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1740 | 小鼠磷酸化肌球蛋白輕鏈(PMLC)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1741 | 小鼠磷酸化*羥化酶(p-TH)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1742 | 小鼠磷酸化糖原合成酶激酶3β(PGSK3β)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1743 | 小鼠磷酸化細胞外信號調節(jié)激酶(pERK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1744 | 小鼠磷酸化細胞外信號調節(jié)激酶(p-ERK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1745 | 小鼠磷酸化腺苷酸活化蛋白激酶(AMPK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1746 | 小鼠磷酸化腺苷酸活化蛋白激酶(p-AMPK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1747 | 小鼠磷酸化血管內皮生長因子受體2(p-VEGFR2)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1748 | 小鼠磷酸肌醇3激酶(PI3K)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1749 | 小鼠磷酸肌酸激酶(CPK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
JW.MO1750 | 小鼠磷酸烯醇式丙酮酸羧激酶(PEPCK)ELISA檢測試劑盒 | 48T/96T | 國產/進口 |
