Description
Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product.
Analysis Note Protein determined by biuret
General description Molecular Weight: 160 kDa （gel filtration）
pI: 4.2
Extinction coefficient: E1% = 16.7 （280 nm）
Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular weight of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing approximay 16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.
Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates.
The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a KM of 33-110 mM.
Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.
Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction.
Quality May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.
Unit Definition One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 °C, equivalent to an O2 uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.
Properties
type Type X-S
form lyophilized powder
composition Protein, ~75%
foreign activity Catalase ≤5 units/mg protein
storage temp. −20°C
Safety
Personal Protective Equipment dust mask type N95 （US）, Eyeshields, Faceshields, Gloves
Hazard Codes Xn
Risk Statements 42
Safety Statements 22-45
WGK Germany 1
RTECS RQ8452000
M0017 葡萄糖氧化酶/GOX/GOD/Glucose oxidase 100-250u/mg 10ku
50ku
250ku
M0018 D-α-氨基氧化酶/D-氨基酸氧化酶/D-AAO 0.5u/mg 100毫克
M0018-1 L-氨基酸氧化酶/L-AAO/TM-LAO/L-amino acid oxidase 4u/mg 2毫克
5毫克
M0019 己糖激酶/己糖磷酸激酶/Hexokinase from Saccharomyces cerevisiae 130-250u/mg 500u
2500u
M0020 胃蛋白酶（豬胃粘膜）/胃液素/胃朊酶/蛋白酵素/酸腈酶/百布圣/Pepsin（porcine stomach mucose） 1：30000 5克
25克
100克
1：15000 25克
100克
1：10000 25克
100克
BR，1：3000 25克
250克
M0021 胰酶粉/胰酵素/胰醇素/胰液素/Pancreatin BR，1：125 250克
BR，1：250 250克
BR，1：4000 100克
500克
M0022 胰蛋白酶（牛胰）/Trypsin（bovine pancreas） 1：250；1000u/mg 25克
100克
1公斤
1：2500；BAEE1萬u/mg 1克
5克
1克
5克
M0023 胰蛋白酶（豬胰）/Trypsin（porcine pancreas） BR；1：250 25克
100克
1公斤
25克
100克
BR；1：300 25克
100克
M0024 脂肪酶（豬胰）/脂酶/脂肪水解酶/脂肪分解酵素/Lipase（pocine pancreas） 3萬u/g 5克
100克
1公斤
M0026 過氧化氫酶（牛肝）/氯化酵素/Catalase（bovine liver） 2000-5000u/mg 1克
50克
1克
5克
25克
30KU/mg 1克
M0028 溶菌酶（蛋清）/脆壁質(zhì)酶/球蛋白G/N-乙酞胞壁質(zhì)聚糖水解酶/Lysozyme（Chinken egg） ＞2萬u/mg 5克
100克
1公斤
5克
M0028-1 溶葡球菌酶/溶葡萄球菌酶/Lysostaphin 1200u/mg 1毫克
M0029 氯化溶菌酶/Lysozyme chloride ~60000u/mg 1克
M0030 纖維素酶（綠色木酶）/1,4-β-D-葡聚糖葡糖苷水解酶/Cellulase（Trichoderma Vride G） 15u/mg 10克
35u/mg 5克
400u/mg 10克
500克
M0032 纖維素酶R-10/1,4-β-D-葡聚糖葡糖苷水解酶/Cellulase Onozuke R-10 10u/mg 1克
5克
10克
50克
100克
M0033 半纖維素酶/Hemicellulase 0.3-3.0u/mg 150ku
400HCu/mg 150ku
M0034 β-葡萄糖苷酸酶/β-葡糖苷酸酶/β-Glucuronidase from bovine liver 3000u/mg，牛肝提取 300ku
M0034-1 β-葡萄糖苷酶/β-糖苷酶/β-D-葡萄糖苷葡萄糖水解酶/龍膽二糖酶/纖維二糖酶/苦杏仁苷酶β-Glucosidase from almonds 30u/mg，杏仁提取 10u
100u
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