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fermentasRNA酶抑制劑RiboLock RNase Inhibitor

Description

Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.

Highlights

Performs under a wide range of reaction conditions
Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)

Note

DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.

Specifications

CategoryName
Concentration	40 U/µL
Definition of Activity Unit	One unit of the RiboLock RNase Inhibitor inhibits the activity of 5 ng RNase A by 50%. Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/mL BSA, 100 ng/mL RNase A, 0.1 mg/mL [³H]-RNA, 50 mg/mL yeast RNA, 8 mM DTT.
Hazardous	No
Hazardous:	No
Inactivation	Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C.
Inhibition	Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound.
Molecular Weight	49.6 kDa monomer
Quality Control	The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests. Functionally tested in RNA and cDNA synthesis.
Shelf Life:
Shipping Condition:
Shipping Information
Source	E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
SpecificationName
SpecificationValue
Storage Buffer	The enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol.
Storage Condition	-20 C
Storage Condition:

fermentasRNA酶抑制劑RiboLock RNase Inhibitor *

*明細(xì)：

DreamTaq Green PCR Master Mix (2×)	K1081	200rxns	~~500~~	300
DreamTaq Green PCR Master Mix (2×)	K1082	1000rxns	~~2,000~~	1200
DreamTaq PCR Master Mix (2×)	K1071	200rxns	~~500~~	300
DreamTaq PCR Master Mix (2×)	K1072	1000rxns	~~2,000~~	1200
PCR master Mix (2×)	K0171	200rxns	~~450~~	276
PCR master Mix (2×)	K0172	1000rxns	~~1,800~~	1080
Long PCR Enzyme Mix	K0181	100U	~~250~~	150
Long PCR Enzyme Mix	K0182	500U	~~1,000~~	600
DreamTaq DNA Polymerase	EP0701	200U	~~150~~	90
	EP0702	500U	~~200~~	120
	EP0703	5x500U	~~900~~	540
	EP0704	20x500U	~~3,200~~	1920
DreamTaq Green DNA Polymerase	EP0711	200U	~~150~~	90
	EP0712	500U	~~200~~	120
	EP0713	5x500U	~~1,080~~	648
	EP0714	20x500U	~~3,200~~	1920
TurboFect in vitro Transfection Reagent	R0531	1ml	~~2,000~~	1300
TurboFect siRNA Transfection Reagent	R1401	0.5ml	~~1,600~~	1040
TurboFect Protein Transfection Reagent	R1411	125ul	~~2,140~~	1391
TurboFect in vivo Transfection Reagent	R0541	120ul	~~3,000~~	1950

“贈(zèng)送送到Hi”

品名	貨號(hào)	包裝	目錄價(jià)￥	贈(zèng)送說(shuō)明
RevertAid First Strand cDNA Synthesis Kit	K1622	100rxns	1,600	買(mǎi)2個(gè)，贈(zèng)1個(gè)K1081（DreamTaq Green PCR Master Mix），價(jià)值￥500
Maxima First Strand cDNA Synthesis Kit	K1641	50rxns	2,500	同時(shí)購(gòu)買(mǎi)，贈(zèng)2個(gè)K1081（DreamTaq Green PCR Master Mix），價(jià)值￥1000
GeneJET RNA Purification Kit	K0731	50preps	1,900
Maxima First Strand cDNA Synthesis Kit	K1642	200rxns	7,500	同時(shí)購(gòu)買(mǎi)，贈(zèng)2個(gè)K1082（DreamTaq Green PCR Master Mix），價(jià)值￥4000
GeneJET RNA Purification Kit	K0732	250preps	7,600
RevertAid Premium First Strand cDNA Synthesis Kit	K1651	50rxns	970	同時(shí)購(gòu)買(mǎi)，贈(zèng)2個(gè)K1081（DreamTaq Green PCR Master Mix），價(jià)值￥1000
GeneJET RNA Purification Kit	K0731	50preps	1,900
RevertAid Premium First Strand cDNA Synthesis Kit	K1652	200rxns	3,880	同時(shí)購(gòu)買(mǎi)，贈(zèng)2個(gè)K1082（DreamTaq Green PCR Master Mix），價(jià)值￥4000
GeneJET RNA Purification Kit	K0732	250preps	7,600
Maxima Reverse Transcriptase	EP0741	2000U	450	買(mǎi)1個(gè)，贈(zèng)1個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥200
Maxima Reverse Transcriptase	EP0742	10000U	1450	買(mǎi)1個(gè)，贈(zèng)2個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥400
RevertAid Premium Reverse Transcriptase	EP0731	2000U	420	買(mǎi)1個(gè)，贈(zèng)1個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥200
RevertAid Premium Reverse Transcriptase	EP0732	10000U	1400	買(mǎi)1個(gè)，贈(zèng)2個(gè)EO0381（RiboLock RNase Inhibitor），價(jià)值￥400

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