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Anti-SRD5A1抗體,類固醇5α還原酶1抗體

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“Anti-SRD5A1抗體,類固醇5α還原酶1抗體"嚴(yán)格質(zhì)控,價格合理,售后服務(wù)完整,任何質(zhì)量問題可包換包退,訂購。!

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博研生物抗體,可應(yīng)用于IHC、WB、 IF、IP、ELISA等實驗,咨詢,質(zhì)量保證,無效免費退換,另外本公司生產(chǎn)經(jīng)營各種標(biāo)記二抗、免疫印跡常用試劑、免疫組化常用試劑,咨詢!

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產(chǎn)品名稱:Anti-SRD5A1抗體,類固醇5α還原酶1抗體

產(chǎn)品編號:BYK-11308R

產(chǎn)品規(guī)格: 0.1ml/0.2ml

抗體來源:Rabbit OR Mouse

產(chǎn)品用途:科研實驗。

性 狀: Lyophilized or Liquid
濃 度: 1mg/1ml
亞 型: IgG

貯 存: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications..

相關(guān)標(biāo)記: HRP Biotin Gold RBITC AP FITC Cy3 Cy5 Cy5.5 Cy7 PE PE-Cy3 PE-CY5 PE-CY5.5 PE-CY7 APC Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647

產(chǎn)品介紹:Steroid 5a-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5a-dihydro-testosterone, which mediates androgen effects on target tissues. The enzyme exists as two isoforms: type 1, which is expressed mainly in the skin; and type 2, which is expressed mainly in the prostate. In cultured human skin cells, 5a-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5a-Reductase 1 is expressed in all portions of the hair follicle, whereas 5a-Reductase 2 is expressed only in mesenchymal portions. In addition, 5a-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5a-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5a-Reductase-3a-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.

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Anti-SRD5A1抗體,類固醇5α還原酶1抗體進行疫組化步驟:

石蠟包埋組織切片3~4μm 厚度
1.烤片: 將待做切片置于切片架上,于60℃恒溫烤箱中至少烤1 hr;
2.脫蠟: 切片放入盛有二甲苯的容器中脫蠟3次(即二甲苯Ⅰ、Ⅱ、Ⅲ),每次10 min;
3.水化: 切片經(jīng)下行酒精水化,無水乙醇5min,95%乙醇2次(每次2min),85%乙醇2 min;75%乙醇2min,自來水沖洗,ddH2O洗2×2min;
4.抗原修復(fù): 根據(jù)抗體說明書*方法進行抗原修復(fù),常采用高壓、微波(溫度達到98~100℃)或酶消化修復(fù)法,室溫自然冷卻,自來水沖洗,ddH2O洗2×2min,TBS洗滌(2×2min)(具體修復(fù)方法見附1)* 注:有些抗原勿需修復(fù),直接進入第5步封閉。
5.封閉: 滴加試劑B,37℃濕盒孵育30 min;
6.加一抗:滴加用試劑C(選擇合理的工作比例),37℃濕盒孵育2 hr 或4℃過夜;
7.洗滌: TBS-T洗滌(3×5 min);
8.封閉: 滴加試劑B,37℃濕盒孵育10 min;
9.加二抗: 滴加用抗體稀釋液稀釋的*化二抗(試劑D),37℃濕盒中孵育30 min;
10.洗滌: TBS-T洗滌(3×5 min);
11.封閉: 滴加試劑Tween 20,37℃濕盒孵育封閉20 min;
12.加HRP-SA: 滴加用試劑C稀釋的試劑E(1:50~200,終濃度5~20 nM),37℃濕盒中孵育30 min;
13.洗滌: TBS-T洗滌(3×5 min),TBS洗滌(2×5 min);
14.顯色:應(yīng)用DAB溶液(試劑F)顯色;
15.復(fù)染:自來水充分沖洗,復(fù)染,脫水,透明;
16.封片: 待組織標(biāo)本干后,用試劑緩沖甘油封固劑封片;
17.觀察成像: 顯微鏡下觀察成像。

Anti-SRD5A1抗體,類固醇5α還原酶1抗體其他優(yōu)勢產(chǎn)品:

Anti-CD82/KAI1/FITC  熒光素標(biāo)記腫瘤轉(zhuǎn)移抑制蛋白1抗體IgG
Anti-CD83/Cell surface protein HB15/FITC  熒光素標(biāo)記CD83抗體IgG
Anti-CD84/SLAMF5/FITC  熒光素標(biāo)記CD84抗體IgG
Anti-CD86/B7-2 /FITC  熒光素標(biāo)記CD86抗體IgG
Anti-CD90/Thy-1/FITC  熒光素標(biāo)記CD90抗體IgG
Anti-ICAM-2/CD102 /FITC  熒光素標(biāo)記細胞間粘附分子-2抗體IgG
Anti-CD105/Endoglin/TGF-β receptor/FITC  熒光素標(biāo)記CD105/轉(zhuǎn)化生長因子β受體抗體IgG
Anti-CD57/FITC  熒光素標(biāo)記抗CD57抗體IgG
Anti-CD58/LFA-3/FITC  熒光素標(biāo)記淋巴細胞功能相關(guān)抗原-3抗體IgG
Anti-CD59/FITC  熒光素標(biāo)記反應(yīng)性溶血膜抑制蛋白抗體IgG
Anti-CD62L/FITC  熒光素標(biāo)記L選擇素抗體IgG
Anti-CD63/MLA1 /FITC  熒光素標(biāo)記抗溶酶體膜糖蛋白抗體IgG
Anti-CD40L /FITC  熒光素標(biāo)記抗CD40L抗體IgG
Anti-CD34/RBITC  紅色熒光素羅丹明(RBITC)標(biāo)記CD34抗體IgG
Anti-CD44/FITC  熒光素標(biāo)記CD44抗體IgG
Anti-CD43/SPN/FITC  熒光素標(biāo)記CD43抗體IgG
Anti-CD44/RBITC  紅色熒光素羅丹明(RBITC)標(biāo)記CD44抗體 IgG
Anti-CD44V6/CD44/HCAM/FITC  熒光素標(biāo)記抗CD44抗體IgG
Anti-CD45(LCA)/FITC  熒光素標(biāo)記CD45(白細胞共同抗原)抗體
Anti-CD45/RBITC  紅色熒光素羅丹明(RBITC)標(biāo)記CD45(白細胞共同抗原)抗體 IgG
Anti-CD45RO/FITC  熒光素標(biāo)記CD45RO抗體IgG
Anti-CD46/MCP /FITC  熒光素標(biāo)記膜輔蛋白/內(nèi)源性輔助因子活性蛋白抗體(人)IgG
Anti-CD46/MCP /FITC  熒光素標(biāo)記膜輔蛋白/內(nèi)源性輔助因子活性蛋白抗體IgG
Anti-CA125/Biotin  *標(biāo)記兔抗人CA125抗原抗體IgG
Anti-CD48/SLAMF2/FITC  熒光素標(biāo)記CD48抗體IgG
Anti-CD49/VLA /FITC  熒光素標(biāo)記CD49(非常晚期抗原)抗體IgG
Anti-CD133 Anti-gen/Biotin  *化造血干細胞抗原CD133抗體
Anti-CD55/DAF/FITC  熒光素標(biāo)記衰變加速因子CD55抗體IgG
Anti-CD56/FITC  熒光素標(biāo)記CD56抗體IgG
Anti-CD56/NCAM1/FITC  熒光素標(biāo)記神經(jīng)細胞粘附分子1抗體IgG

關(guān)鍵詞:顯微鏡
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