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anti-P53 抗體說(shuō)明書

2011年11月18日 09:10:11人氣:1407來(lái)源:上海瑞齊生物科技有限公司

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anti-P53 抗體說(shuō)明書

Quantity size:  0.2ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)

 Background: The widely studied P53 tumor suppressor gene has been found to contain mutations in over 50% of human cancers. The level of P53 protein is low in normal cells, but is increased in response to DNA damage or various other cellular distress signals. Overexpression of the P53 transcription factor can induce ether cell cycle arrest or apoptosis through transcriptional regulation of several genes including the cell cycle ingibitor P21, DNA repair gene GADD45, and the apoptotic inducer Bax. P53 has also been shown to induce apoptosis by meas of s direct signaling pathway. P53 directly binds to and acts on several cellular protein involved in various pathway, including c-Abl, basal transcription factor TFIIH and WT1. P53 can be functionally inactivated by mutation, binding to DNA tumor virus encoded proteins, such as SV40 large T antigen, Adenovirus E1B and papilloma virus E6 proteins or as a consequence of its interaction with the oncogene-encoded protein MDM2.
 

anti-P53 抗體說(shuō)明書

Specificity:     · anti-P53 is a rabbit monoclonal antibody unconjugated
· specific for P53 of human, mouse, rat and chicken
· epitope mapping near the amino terminus of P53 of
    human, mouse, rat and chicken origin  
· use for western blotting, elisa, immunoprecipitation and  
    immunohistochemistry
· affinity chromatography purification, purity :>95%
· Isotype: IgG 
· mol wt: 110kDa  
 
Application:    · Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000 
· Optimal working dilutions must be determined by the end user.
 
 

anti-P53 抗體說(shuō)明書

Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

上海瑞齊生物科技有限公司作者

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