Description
Application SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem （Pharmacia） and Schagger and von Jagow SDS-PAGE （Tricine）. SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximay equal intensity after electrophoresis and Coomassie blue staining.
Other Notes Protein （mol. wt.）
Aprotinin, bovine lung （6,500）
α-Lactalbumin, bovine milk （14,200）
Trypsin inhibitor, soybean （20,000）
Trypsinogen, bovine pancreas （24,000）
Carbonic anhydrase, bovine erythrocytes （29,000）
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle （36,000）
Ovalbumin, chicken egg （45,000）
Albumin, bovine serum （66,000）
Will yield eight protein bands with Coomassie Blue staining.
Reconstitution Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 0.5% dithiothreitol, 2% SDS, and 0.0005% bromophenol blue.
Legal Information SigmaMarker is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Properties
form lyophilized powder
usage sufficient for 10-20 large gels （16 × 18 cm）
sufficient for 20-30 mini-gels （10 × 10 cm）
vial sufficient for 50-100 PhastGels
mol wt mol wt 6,500-66,000 Da
storage temp. 2-8°C
Safety
Hazard Codes Xn
Risk Statements 42/43
Safety Statements 36
WGK Germany 3
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