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Rabbit anti-NOTCH 1

2010年10月14日 08:37:12人氣:790來(lái)源:上海瑞齊生物科技有限公司

 

Rabbit anti-NOTCH 1
Catalog Number :   RS-1335R   
 
Quantity size :   0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )
 
Background:    Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
Specificity :     ·anti-NOTCH 1 is a rabbit polyclonal antibody unconjugated
                 ·specific for NOTCH 1 of human, mouse and rat 
                 ·epitope mapping near the amino terminus of NOTCH 1 of
                   human, mouse rat, pig origin    
                 ·use for western blotting, elisa, immunoprecipitation  
                   and immunohistochemistry
· affinity chromatography purification, purity :>95%
· Isotype: IgG    
· mol wt: 270kDa
 
Application:   ·Western blotting 1:100-500
                 · Immunohistochemistry 1:100-500
                 ·ELISA 1:500-1000
   · Optimal working dilutions must be determined by the end user.
 
Storage:    Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in
human, therapeutic or diagnostic applications.
 
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